MetaCyc Pathway: anandamide biosynthesis I in Escherichia coli BW25113

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Reactions and Genes LB Anaerobic with Benzethonium chloride 0.000015625 mM
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Glycerol-3-phosphate O-acyltransferase:
arachidonoyl-CoA + sn-glycerol 3-phosphate→1-arachidinoyl-sn-glycerol 3-phosphate + coenzyme A (EC 2.3.1.15)
b1090 (plsX) +0.0
b3059 (ygiH) -0.2
b4041 (plsB) -0.5
1-acylglycerol-3-phosphate O-acyltransferase:
1-arachidinoyl-sn-glycerol 3-phosphate + an acyl-CoA→a 1-arachidonoyl-2-acyl-sn-glycerol 3-phosphate + coenzyme A (EC 2.3.1.51)
b3018 (plsC) N.D.
b3862 (yihG) -0.1
Phosphatidate phosphatase:
a 1-arachidonoyl-2-acyl-sn-glycerol 3-phosphate + H2O→a 1-arachidonoyl-2-acyl-sn-glycerol + phosphate (EC 3.1.3.4)
No genes
Diacylglycerol cholinephosphotransferase:
a 1-arachidonoyl-2-acyl-sn-glycerol + CDP-choline→a 1-arachidonoyl-2-acyl-sn-glycero-3-phosphocholine + CMP + H+ (EC 2.7.8.2)
No genes
a 1-arachidonoyl-2-acyl-sn-glycero-3-phosphocholine + a phosphatidylethanolamine→an N-arachidonoyl-phosphatidylethanolamine + a 1-lysophosphatidylcholine + H+ (EC 2.3.1.M82)
No genes
an N-arachidonoyl-phosphatidylethanolamine + H2O→anandamide + a phosphatidate + H+ (EC 3.1.4.54)
No genes
Phospholipase A(2):
an N-arachidonoyl-phosphatidylethanolamine + H2O→an N-arachidonoyl-2-lysophosphatidylethanolamine + a fatty acid + H+ (EC 3.1.1.4)
No genes
Phospholipase A(1):
an N-arachidonoyl-phosphatidylethanolamine + H2O→an N-arachidonoyl-1-lysophosphatidylethanolamine + a fatty acid + H+ (EC 3.1.1.32)
b3821 (pldA) -1.2
Alkylglycerophosphoethanolamine phosphodiesterase:
an N-arachidonoyl-2-lysophosphatidylethanolamine + H2O→anandamide + a 2-lysophosphatidate + H+ (EC 3.1.4.39)
No genes
Phospholipase A(2):
an N-arachidonoyl-2-lysophosphatidylethanolamine + H2O→sn-glycero-3-phospho-N-(arachidonoyl)ethanolamine + a fatty acid + H+ (EC 3.1.1.4)
No genes
Phospholipase A(2):
an N-arachidonoyl-1-lysophosphatidylethanolamine + H2O→sn-glycero-3-phospho-N-(arachidonoyl)ethanolamine + a fatty acid + H+ (EC 3.1.1.4)
No genes
Glycerophosphodiester phosphodiesterase:
sn-glycero-3-phospho-N-(arachidonoyl)ethanolamine + H2O→anandamide + sn-glycerol 3-phosphate + H+ (EC 3.1.4.46)
b2239 (glpQ) -0.3
b3449 (ugpQ) +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information