MetaCyc Pathway: stearate biosynthesis IV in Escherichia coli BW25113

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Reactions and Genes LB Aerobic with Irgasan 0.0000003125 mM
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Beta-ketoacyl-acyl-carrier-protein synthase I:
a palmitoyl-[acp] + a malonyl-[acp] + H+→a 3-oxooctadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.41)
b2323 (fabB) N.D.
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxooctadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyoctadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
b1093 (fabG) N.D.
b2137 (yohF) +0.2
b2426 (ucpA) -0.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyoctadecanoyl-[acp]→a (2E)-octadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
b0180 (fabZ) N.D.
b0954 (fabA) N.D.
a (2E)-octadec-2-enoyl-[acp] + NADPH + H+→a stearoyl-[acp] + NADP+ (EC 1.3.1.104)
No genes
Oleoyl-[acyl-carrier-protein] hydrolase:
a stearoyl-[acp] + H2O→stearate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
Long-chain-fatty-acid--CoA ligase:
stearate + ATP + coenzyme A→stearoyl-CoA + AMP + diphosphate (EC 6.2.1.3)
b1805 (fadD) +7.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information