MetaCyc Pathway: superpathway of coenzyme A biosynthesis I (bacteria) in Escherichia coli BW25113

Reactions and Genes	LB Aerobic with Irgasan 0.0000003125 mM
3-methyl-2-oxobutanoate hydroxymethyltransferase: 3-methyl-2-oxobutanoate + a 5,10-methylenetetrahydrofolate + H2O→2-dehydropantoate + a tetrahydrofolate (EC 2.1.2.11)
b0134 (panB)	+1.7
Aspartate 1-decarboxylase: L-aspartate + H+→β-alanine + CO2 (EC 4.1.1.11)
b0131 (panD)	+2.0
2-dehydropantoate 2-reductase (in reverse): 2-dehydropantoate + NADPH + H+→(R)-pantoate + NADP+ (EC 1.1.1.169)
b0425 (panE)	+1.0
b3774 (ilvC)	-0.0
Pantoate--beta-alanine ligase: β-alanine + (R)-pantoate + ATP→(R)-pantothenate + AMP + diphosphate + H+ (EC 6.3.2.1)
b0133 (panC)	+1.3
Pantothenate kinase: (R)-pantothenate + ATP→(R)-4'-phosphopantothenate + ADP + H+ (EC 2.7.1.33)
b3974 (coaA)	N.D.
Phosphopantothenate--cysteine ligase: (R)-4'-phosphopantothenate + CTP + L-cysteine→(R)-4'-phosphopantothenoyl-L-cysteine + CMP + diphosphate + H+ (EC 6.3.2.5)
b3639 (dfp)	N.D.
Phosphopantothenoylcysteine decarboxylase: (R)-4'-phosphopantothenoyl-L-cysteine + H+→4'-phosphopantetheine + CO2 (EC 4.1.1.36)
b3639 (dfp)	N.D.
Pantetheine-phosphate adenylyltransferase: 4'-phosphopantetheine + ATP + H+→3'-dephospho-CoA + diphosphate (EC 2.7.7.3)
b3634 (coaD)	N.D.
Dephospho-CoA kinase: 3'-dephospho-CoA + ATP→coenzyme A + ADP + H+ (EC 2.7.1.24)
b0103 (coaE)	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information