MetaCyc Pathway: (8E,10E)-dodeca-8,10-dienol biosynthesis in Escherichia coli BW25113

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Reactions and Genes Dodecanol (C)
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palmitoyl-CoA[in] + an oxidized electron-transfer flavoprotein[in] + H+[in]→(2E)-hexadec-2-enoyl-CoA[in] + a reduced electron-transfer flavoprotein[in] (EC 1.3.8.9)
No genes
(8E,10E)-dodeca-8,10-dienoate + 2 NADPH + 3 H+→(8E,10E)-dodeca-8,10-dienol + 2 NADP+ + H2O
No genes
Enoyl-CoA hydratase (in reverse):
(2E)-hexadec-2-enoyl-CoA + H2O→(S)-3-hydroxyhexadecanoyl-CoA (EC 4.2.1.17)
b1393 (paaF) N.D.
b1394 (paaG) -0.0
b2341 (yfcX) -0.2
b3846 (fadB) -1.4
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxyhexadecanoyl-CoA + NAD+→3-oxopalmitoyl-CoA + NADH + H+ (EC 1.1.1.211)
b2341 (yfcX) -0.2
b3846 (fadB) -1.4
Acetyl-CoA C-myristoyltransferase (in reverse):
3-oxopalmitoyl-CoA + coenzyme A→myristoyl-CoA + acetyl-CoA (EC 2.3.1.155)
No genes
myristoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-tetradec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Enoyl-CoA hydratase (in reverse):
(2E)-tetradec-2-enoyl-CoA + H2O→(S)-3-hydroxytetradecanoyl-CoA (EC 4.2.1.17)
b1393 (paaF) N.D.
b1394 (paaG) -0.0
b2341 (yfcX) -0.2
b3846 (fadB) -1.4
(S)-3-hydroxytetradecanoyl-CoA + NAD+→3-oxomyristoyl-CoA + NADH + H+
b2341 (yfcX) -0.2
b3846 (fadB) -1.4
Acetyl-CoA C-acyltransferase (in reverse):
3-oxomyristoyl-CoA + coenzyme A→lauroyl-CoA + acetyl-CoA (EC 2.3.1.16)
b1397 (paaJ) -0.1
b2224 (atoB) +0.1
b2342 (yfcY) -0.2
b2844 (yqeF) -0.0
b3845 (fadA) -0.5
lauroyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(9E)-dodecenoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes
(9E)-dodecenoyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(8E,10E)-dodeca-8,10-dienoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information