MetaCyc Pathway: methyl ketone biosynthesis (engineered) in Escherichia coli BW25113
Reactions and Genes | Dodecanol (C) |
---|---|
remove | |
a fatty acid + ATP + coenzyme A→an acyl-CoA + AMP + diphosphate | |
No genes | |
Acyl-CoA oxidase: a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide (EC 1.3.3.6) | |
No genes | |
Enoyl-CoA hydratase (in reverse): a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA (EC 4.2.1.17) | |
b1393 (paaF) | N.D. |
b1394 (paaG) | -0.0 |
b2341 (yfcX) | -0.2 |
b3846 (fadB) | -1.4 |
3-hydroxyacyl-CoA dehydrogenase: a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35) | |
b2341 (yfcX) | -0.2 |
b3846 (fadB) | -1.4 |
a 2,3,4-saturated 3-oxoacyl-CoA + H2O→a 3-oxoacid + coenzyme A + H+ | |
No genes | |
a 3-oxoacid + H+→a methyl ketone + CO2 (spontaneous) | |
No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information