MetaCyc Pathway: superpathway of phenylethylamine degradation in Escherichia coli BW25113

Reactions and Genes	Dodecanol (C)
Primary-amine oxidase: 2-phenylethylamine + dioxygen + H2O→phenylacetaldehyde + ammonium + hydrogen peroxide (EC 1.4.3.21; 1.4.3.4)
b1386 (tynA)	+0.2
Phenylacetaldehyde dehydrogenase: phenylacetaldehyde + NAD+ + H2O→phenylacetate + NADH + 2 H+ (EC 1.2.1.39)
b1385 (feaB)	+0.4
Phenylacetate--CoA ligase: phenylacetate + ATP + coenzyme A→phenylacetyl-CoA + AMP + diphosphate (EC 6.2.1.30)
b1398 (paaK)	N.D.
phenylacetyl-CoA + NADPH + dioxygen + H+→2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + NADP+ + H2O (EC 1.14.13.149)
b1388 (paaA)	+0.3
b1389 (paaB)	N.D.
b1390 (paaC)	+0.1
b1392 (paaE)	+0.0
2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA→2-oxepin-2(3H)-ylideneacetyl-CoA (EC 5.3.3.18)
b1394 (paaG)	-0.0
2-oxepin-2(3H)-ylideneacetyl-CoA + H2O→3-oxo-5,6-didehydrosuberyl-CoA semialdehyde (EC 3.3.2.12)
b1387 (maoC)	-0.1
3-oxo-5,6-didehydrosuberyl-CoA semialdehyde + NADP+ + H2O→3-oxo-5,6-didehydrosuberyl-CoA + NADPH + 2 H+ (EC 1.2.1.91)
b1387 (maoC)	-0.1
3-oxo-5,6-didehydrosuberyl-CoA + coenzyme A→(2E)-5-carboxy-2-pentenoyl-CoA + acetyl-CoA (EC 2.3.1.223)
b1397 (paaJ)	-0.1
Enoyl-CoA hydratase (in reverse): (2E)-5-carboxy-2-pentenoyl-CoA + H2O→(3S)-hydroxyadipyl-CoA (EC 4.2.1.17)
b1393 (paaF)	N.D.
b1394 (paaG)	-0.0
b2341 (yfcX)	-0.2
b3846 (fadB)	-1.4
3-hydroxyacyl-CoA dehydrogenase: (3S)-hydroxyadipyl-CoA + NAD+→3-oxoadipoyl-CoA + NADH + H+ (EC 1.1.1.35)
b1395 (paaH)	+0.2
b2341 (yfcX)	-0.2
b3846 (fadB)	-1.4
3-oxoadipyl-CoA thiolase (in reverse): 3-oxoadipoyl-CoA + coenzyme A→succinyl-CoA + acetyl-CoA (EC 2.3.1.174)
b1397 (paaJ)	-0.1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information