MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Pseudomonas putida KT2440

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Reactions and Genes 3-methyl-3-buten-1-ol 0.25 vol% (C)
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
PP_1157 N.D.
PP_1394 +0.1
PP_3365 -0.1
PP_3723 -0.3
PP_4679 -5.0
PP_4680 -5.2
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
PP_4678 -6.2
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
PP_5128 -4.7
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
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Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
PP_1616 -1.9
PP_2049 +0.4
PP_2426 -0.3
PP_2650 +0.3
PP_2803 -0.1
PP_3839 -0.0
PP_4760 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information