MetaCyc Pathway: superpathway of aromatic amino acid biosynthesis in Pseudomonas simiae WCS417

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Reactions and Genes LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
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3-deoxy-7-phosphoheptulonate synthase:
D-erythrose 4-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (EC 2.5.1.54)
PS417_08430 -1.0
PS417_08525 -0.0
PS417_10705 -0.7
3-dehydroquinate synthase:
3-deoxy-D-arabino-heptulosonate 7-phosphate→3-dehydroquinate + phosphate (EC 4.2.3.4)
PS417_01985 N.D.
3-dehydroquinate dehydratase:
3-dehydroquinate→3-dehydroshikimate + H2O (EC 4.2.1.10)
PS417_06310 N.D.
PS417_18950 +0.1
PS417_24430 +0.4
PS417_02975 -0.2
Shikimate dehydrogenase (in reverse):
3-dehydroshikimate + NADPH + H+→shikimate + NADP+ (EC 1.1.1.25)
PS417_10420 +0.2
PS417_00125 N.D.
PS417_24435 -0.1
Shikimate kinase:
shikimate + ATP→shikimate 3-phosphate + ADP + H+ (EC 2.7.1.71)
PS417_01980 N.D.
3-phosphoshikimate 1-carboxyvinyltransferase:
shikimate 3-phosphate + phosphoenolpyruvate→5-enolpyruvoyl-shikimate 3-phosphate + phosphate (EC 2.5.1.19)
PS417_08135 -1.3
PS417_12855 -4.3
Chorismate synthase:
5-enolpyruvoyl-shikimate 3-phosphate→chorismate + phosphate (EC 4.2.3.5)
PS417_19315 N.D.
Anthranilate synthase:
chorismate + L-glutamine→anthranilate + L-glutamate + H+ + pyruvate (EC 4.1.3.27)
PS417_25720 -0.7
PS417_25725 -1.1
Chorismate mutase:
chorismate→prephenate (EC 5.4.99.5)
PS417_08125 -5.3
PS417_01650 -0.2
Prephenate dehydratase:
prephenate + H+→3-phenyl-2-oxopropanoate + CO2 + H2O (EC 4.2.1.51)
PS417_07360 -0.4
PS417_08125 -5.3
Prephenate dehydrogenase:
prephenate + NAD+→3-(4-hydroxyphenyl)pyruvate + CO2 + NADH (EC 1.3.1.12)
PS417_08135 -1.3
Anthranilate phosphoribosyltransferase (in reverse):
anthranilate + 5-phospho-α-D-ribose 1-diphosphate→N-(5-phosphoribosyl)-anthranilate + diphosphate (EC 2.4.2.18)
PS417_16455 +0.3
PS417_25715 -1.2
Phosphoribosylanthranilate isomerase:
N-(5-phosphoribosyl)-anthranilate→1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate (EC 5.3.1.24)
PS417_18560 -2.6
Aspartate transaminase (in reverse):
3-phenyl-2-oxopropanoate + L-glutamate→L-phenylalanine + 2-oxoglutarate (EC 2.6.1.1; 2.6.1.27; 2.6.1.57)
PS417_08130 -0.2
PS417_08560 -0.0
PS417_09070 +0.0
PS417_09120 +0.0
PS417_11840 +0.0
PS417_12775 -0.0
PS417_12985 -0.3
PS417_13545 +0.6
PS417_14865 +0.2
PS417_15040 -0.2
PS417_15990 +0.3
PS417_18690 -0.8
PS417_19025 +0.0
PS417_19385 -0.0
PS417_19910 -0.6
PS417_21030 -0.3
PS417_21275 +3.0
PS417_23120 -0.3
PS417_23365 -0.3
PS417_27730 -0.0
PS417_28165 -0.1
PS417_04425 +0.2
Aspartate transaminase (in reverse):
3-(4-hydroxyphenyl)pyruvate + L-glutamate→L-tyrosine + 2-oxoglutarate (EC 2.6.1.1; 2.6.1.27; 2.6.1.5; 2.6.1.57)
PS417_08130 -0.2
PS417_08560 -0.0
PS417_09070 +0.0
PS417_09120 +0.0
PS417_11840 +0.0
PS417_12775 -0.0
PS417_12985 -0.3
PS417_13545 +0.6
PS417_14865 +0.2
PS417_15040 -0.2
PS417_15990 +0.3
PS417_18690 -0.8
PS417_19025 +0.0
PS417_19385 -0.0
PS417_19910 -0.6
PS417_21030 -0.3
PS417_21275 +3.0
PS417_23120 -0.3
PS417_23365 -0.3
PS417_27730 -0.0
PS417_28165 -0.1
PS417_04425 +0.2
Indole-3-glycerol-phosphate synthase:
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H+→(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O (EC 4.1.1.48)
PS417_25710 -2.3
Indole-3-glycerol-phosphate lyase:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate→indole + D-glyceraldehyde 3-phosphate (EC 4.1.2.8; 4.2.1.20)
PS417_00180 -3.4
PS417_00185 -4.5
PS417_04445 -0.1
indole + L-serine→L-tryptophan + H2O (EC 4.2.1.122; 4.2.1.20)
PS417_00180 -3.4
PS417_00185 -4.5
PS417_04445 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information