MetaCyc Pathway: Bifidobacterium shunt in Escherichia coli BL21

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Reactions and Genes Bas49
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Hexokinase:
D-glucopyranose + ATP→D-glucopyranose 6-phosphate + ADP + H+ (EC 2.7.1.1; 2.7.1.2)
ECD_00343 -0.6
ECD_02298 +0.6
Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
ECD_02691 -0.4
ECD_03897 -0.8
Fructose-6-phosphate phosphoketolase:
β-D-fructofuranose 6-phosphate + phosphate→acetyl phosphate + D-erythrose 4-phosphate + H2O (EC 4.1.2.22)
No genes
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
ECD_01820 +1.0
ECD_02221 N.D.
ECD_02351 -0.2
ECD_02352 +0.0
ECD_02982 +0.1
Transaldolase (in reverse):
D-erythrose 4-phosphate + β-D-fructofuranose 6-phosphate→D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate (EC 2.2.1.2)
ECD_00008 -0.6
ECD_00792 -0.9
ECD_02355 +0.4
ECD_03832 -0.0
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
ECD_02356 +0.1
ECD_02765 +0.2
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
ECD_02745 N.D.
ECD_03962 +0.3
Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)
ECD_03238 +0.1
ECD_04166 +0.2
Phosphoketolase:
D-xylulose 5-phosphate + phosphate→acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O (EC 4.1.2.9)
No genes
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
ECD_01371 N.D.
ECD_01748 N.D.
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
ECD_02757 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
ECD_00708 +0.2
ECD_03470 +0.0
ECD_04271 -0.5
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
ECD_02624 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
ECD_01645 -2.3
ECD_01825 +0.8
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
ECD_00262 +2.7
ECD_00263 +2.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information