MetaCyc Pathway: acetyl-CoA fermentation to butanoate in Pseudomonas putida KT2440

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Reactions and Genes Vanillic Acid (C)
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Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PP_0582 +0.1
PP_1377 -2.9
PP_2051 -0.2
PP_2137 +0.2
PP_2215 -1.1
PP_3280 -0.2
PP_3355 +0.2
PP_3754 -0.2
PP_4636 +0.3
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
PP_0774 +0.0
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
PP_1457 -0.1
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
No genes
3-hydroxybutyryl-CoA dehydratase:
(3R)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.55)
PP_3270 -0.1
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Propionate CoA-transferase:
acetate + butanoyl-CoA→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information