MetaCyc Pathway: superpathway of L-alanine fermentation (Stickland reaction) in Escherichia coli BW25113

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Reactions and Genes D-Cycloserine 0.1 mg/ml
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Alanine transaminase:
L-alanine + 2-oxoglutarate→pyruvate + L-glutamate (EC 2.6.1.2)
b2290 (yfbQ) -3.3
b2379 (yfdZ) -1.3
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
b1380 (ldhA) -0.1
b2133 (dld) +0.0
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
b1378 (ydbK) +0.0
Propionate CoA-transferase:
(R)-lactate + propanoyl-CoA→(R)-lactoyl-CoA + propanoate (EC 2.8.3.1)
No genes
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
b2297 (pta) -1.3
b2458 (eutI) -0.1
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT) +0.2
b2296 (ackA) N.D.
b2460 (eutQ) +0.1
b2461 (eutP) +0.1
b3115 (tdcD) -0.0
Lactoyl-CoA dehydratase:
(R)-lactoyl-CoA→acryloyl-CoA + H2O (EC 4.2.1.54)
No genes
acryloyl-CoA + NADH + H+→propanoyl-CoA + NAD+ (EC 1.3.1.95)
No genes
Propionate CoA-transferase (in reverse):
propanoyl-CoA + acetate→propanoate + acetyl-CoA (EC 2.8.3.1)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information