| Reactions and Genes | Ying_all64 rep A; time point 3 |
|---|
| remove |
Ent-copalyl diphosphate synthase:
geranylgeranyl diphosphate→(-)-ent-copalyl diphosphate (EC 5.5.1.13) |
| No genes |
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4) |
| ABIE41_RS20090 | N.D. |
| a sulfur-carrier protein with a C-terminal Gly-Gly + ATP + H+→a carboxy-adenylated [sulfur-carrier protein with a C-terminal Gly-Gly] + diphosphate |
| No genes |
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11) |
| ABIE41_RS08445 | N.D. |
| a carboxy-adenylated [sulfur-carrier protein with a C-terminal Gly-Gly] + a reduced two electron carrier + a sulfurated [sulfur carrier]→a thiocarboxylated [sulfur-carrier protein with a C-terminal Gly-Gly] + AMP + an oxidized electron carrier + H+ + an unsulfurated [sulfur carrier] |
| No genes |
| (-)-ent-copalyl diphosphate + H2O→(16R)-ent-kauran-16-ol + diphosphate |
| No genes |
| L-aspartate 4-semialdehyde + glycerone phosphate→2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate (EC 4.1.2.56) |
| No genes |
| (16R)-ent-kauran-16-ol→(16R)-ent-kauran-16,19-diol |
| No genes |
| 2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate→3-amino-4-hydroxybenzoate + H+ + phosphate + 2 H2O (EC 4.1.99.20) |
| No genes |
| (16R)-ent-kauran-16,19-diol→(16R)-hydroxy-ent-kauran-19-oate |
| No genes |
| 3-amino-4-hydroxybenzoate + NADPH + dioxygen + H+→3-amino-2,4-dihydroxybenzoate + NADP+ + H2O (EC 1.14.13.249) |
| No genes |
| (16R)-hydroxy-ent-kauran-19-oate + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→(11S,16S)-ent-kauran-11,16-epoxy-19-oate + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O |
| No genes |
| 3-amino-2,4-dihydroxybenzoate + ATP + coenzyme A→3-amino-2,4-dihydroxybenzoyl-CoA + AMP + diphosphate |
| No genes |
| (11S,16S)-ent-kauran-11,16-epoxy-19-oate + 2-oxoglutarate + dioxygen→(7S,11S,16S)-7-hydroxy-ent-kauran-11,16-epoxy-19-oate + CO2 + succinate |
| No genes |
| 3-amino-2,4-dihydroxybenzoyl-CoA + a thiocarboxylated [sulfur-carrier protein with a C-terminal Gly-Gly] + H2O→a sulfur-carrier protein with a C-terminal Gly-Gly + 3-amino-2,4-dihydroxybenzoylsulfanide + coenzyme A + 2 H+ |
| No genes |
| (7S,11S,16S)-7-hydroxy-ent-kauran-11,16-epoxy-19-oate + NAD+→(11S,16S)-7-oxo-ent-kauran-11,16-epoxy-19-oate + NADH + H+ |
| No genes |
| (11S,16S)-7-oxo-ent-kauran-11,16-epoxy-19-oate + NADPH + H+→(7R,11S,16S)-7-hydroxy-ent-kauran-11,16-epoxy-19-oate + NADP+ |
| No genes |
| (7R,11S,16S)-7-hydroxy-ent-kauran-11,16-epoxy-19-oate + ATP + H+→(7R,11S,16S)-7-hydroxy-ent-kauran-11,16-epoxy-19-oyl-AMP + diphosphate |
| No genes |
| (7R,11S,16S)-7-hydroxy-ent-kauran-11,16-epoxy-19-oyl-AMP + coenzyme A→(7R,11S,16S)-7-hydroxy-ent-kauran-11,16-epoxy-19-oyl-CoA + AMP + H+ |
| No genes |
| (7R,11S,16S)-7-hydroxy-ent-kauran-11,16-epoxy-19-oyl-CoA + an oxidized electron carrier→(4S)-homoplatensicyl-CoA + a reduced two electron carrier |
| No genes |
| (4S)-homoplatensicyl-CoA + an oxidized electron-transfer flavoprotein + H+→homoplatensic-4-enyl-CoA + a reduced electron-transfer flavoprotein |
| No genes |
Enoyl-CoA hydratase (in reverse):
homoplatensic-4-enyl-CoA + H2O→(3S)-3-hydroxy-homoplatensic-CoA (EC 4.2.1.17) |
| ABIE41_RS04195 | -0.2 |
| ABIE41_RS07775 | -0.1 |
| ABIE41_RS07780 | -0.1 |
| ABIE41_RS10405 | -0.1 |
| ABIE41_RS16295 | +0.2 |
| ABIE41_RS17285 | -0.7 |
| ABIE41_RS18700 | +0.8 |
| ABIE41_RS19240 | +0.1 |
| ABIE41_RS19820 | -1.0 |
| ABIE41_RS20600 | N.D. |
| ABIE41_RS23310 | -0.2 |
3-hydroxyacyl-CoA dehydrogenase:
(3S)-3-hydroxy-homoplatensic-CoA + NAD+→3-oxo-homoplatensic-CoA + NADH + H+ (EC 1.1.1.35) |
| ABIE41_RS07780 | -0.1 |
| ABIE41_RS12780 | -0.0 |
| ABIE41_RS18595 | -0.1 |
| ABIE41_RS23310 | -0.2 |
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-homoplatensic-CoA + coenzyme A→platensicyl-CoA + propanoyl-CoA (EC 2.3.1.16) |
| ABIE41_RS07785 | -2.0 |
| ABIE41_RS07970 | +0.2 |
| ABIE41_RS08710 | N.D. |
| ABIE41_RS23315 | -0.1 |
| 3-amino-2,4-dihydroxybenzoylsulfanide + platensicyl-CoA + H+→thioplatensimycin + coenzyme A |
| No genes |
| thioplatensimycin + H2O→platensimycin + hydrogen sulfide + H+ (spontaneous) |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information