MetaCyc Pathway: glycolysis V (Pyrococcus) in Escherichia coli BW25113

Reactions and Genes	Ying_Nucleobase14 rep A; time point 3
ADP-specific glucokinase: D-glucopyranose + ADP→D-glucopyranose 6-phosphate + AMP + H+ (EC 2.7.1.147)
No genes
Glucose-6-phosphate isomerase: α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
b2843 (kduI)	+0.0
b4025 (pgi)	+0.2
ADP-specific phosphofructokinase: β-D-fructofuranose 6-phosphate + ADP→β-D-fructofuranose 1,6-bisphosphate + AMP + H+ (EC 2.7.1.146)
No genes
Fructose-bisphosphate aldolase: β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
b2097 (b2097)	+0.2
b2925 (fbaA)	N.D.
Glyceraldehyde-3-phosphate dehydrogenase (ferredoxin): D-glyceraldehyde 3-phosphate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O→3-phospho-D-glycerate + 3 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.6)
No genes
Triose-phosphate isomerase: D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1)
b3919 (tpiA)	N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
b0755 (gpmA)	-1.4
b3612 (gpmI)	-0.1
b4395 (ytjC)	-1.6
Phosphopyruvate hydratase: 2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
b2779 (eno)	N.D.
Pyruvate kinase (in reverse): phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF)	-3.2
b1854 (pykA)	-0.4
Pyruvate, water dikinase (in reverse): phosphoenolpyruvate + AMP + 2 H+ + phosphate→pyruvate + ATP + H2O (EC 2.7.9.2)
b1702 (pps)	+0.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information