MetaCyc Pathway: thiazole component of thiamine diphosphate biosynthesis II in Escherichia coli BW25113

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Reactions and Genes Ying_AminoAcid20 rep A; time point 3
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1-deoxy-D-xylulose-5-phosphate synthase:
D-glyceraldehyde 3-phosphate + H+ + pyruvate→1-deoxy-D-xylulose 5-phosphate + CO2 (EC 2.2.1.7)
b0420 (dxs) N.D.
Glycine oxidase:
glycine + dioxygen→2-iminoacetate + hydrogen peroxide + H+ (EC 1.4.3.19; 1.4.3.3)
No genes
a [ThiS sulfur-carrier protein] + ATP + H+→a carboxy-adenylated-[ThiS sulfur-carrier protein] + diphosphate (EC 2.7.7.73)
b3992 (thiF) +0.1
Cysteine desulfurase:
L-cysteine + an [L-cysteine desulfurase]-L-cysteine→an [L-cysteine desulfurase]-S-sulfanyl-L-cysteine + L-alanine (EC 2.8.1.7)
b1680 (sufS) -1.5
b2810 (csdA) -0.2
b2811 (ygdK) -0.2
b2530 (iscS) N.D.
Cysteine desulfurase:
a carboxy-adenylated-[ThiS sulfur-carrier protein] + an [L-cysteine desulfurase]-S-sulfanyl-L-cysteine + a reduced two electron carrier→a thiocarboxylated-[ThiS sulfur-carrier protein] + AMP + an oxidized electron carrier + an [L-cysteine desulfurase]-L-cysteine + 2 H+ (EC 2.8.1.7)
b1680 (sufS) -1.5
b2810 (csdA) -0.2
b2811 (ygdK) -0.2
b2530 (iscS) N.D.
2-iminoacetate + 1-deoxy-D-xylulose 5-phosphate + a thiocarboxylated-[ThiS sulfur-carrier protein]→a [ThiS sulfur-carrier protein] + 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 2 H2O (EC 2.8.1.10)
b3991 (thiG) -0.0
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate→2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate (EC 5.3.99.10)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information