MetaCyc Pathway: mixed acid fermentation in Escherichia coli BW25113

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Reactions and Genes Ying_AminoAcid20 rep A; time point 3
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
b3956 (ppc) -0.0
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF) +0.2
b1854 (pykA) -0.2
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
b1380 (ldhA) +0.6
b2133 (dld) +0.0
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC) +0.2
b1479 (sfcA) +0.1
b3236 (mdh) -7.0
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
b0823 (ybiW) +0.0
b0824 (ybiY) -0.1
b0902 (pflA) -0.2
b0903 (pflB) -0.1
b2579 (yfiD) +0.1
b3951 (pflD) -0.1
b3952 (pflC) -0.0
b3114 (tdcE) -0.0
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
b0351 (mhpF) +0.1
b1241 (adhE) +0.1
b2455 (eutE) +0.0
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
b0720 (gltA) -2.4
formate + H+→CO2 + H2
b2483 (hyfC) -0.2
b2722 (hycD) +0.0
b2723 (hycC) -0.1
b2724 (hycB) +0.0
b4079 (fdhF) -0.0
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
b1611 (fumC) -1.5
b1612 (fumA) +0.0
b1675 (ydhZ) -0.5
b2929 (yggD) +0.0
b4122 (fumB) -0.2
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
b2297 (pta) -0.8
b2458 (eutI) -0.1
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT) -0.4
b2296 (ackA) N.D.
b2460 (eutQ) -0.0
b2461 (eutP) +0.0
b3115 (tdcD) +0.0
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
b0118 (acnB) -0.2
b0771 (ybhJ) -0.1
b1276 (acnA) -0.7
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
b0325 (yahK) +0.1
b0356 (frmA) +0.0
b1241 (adhE) +0.1
b1478 (adhP) -0.5
b3253 (yhdH) +0.1
b4269 (yjgB) +0.2
b3589 (yiaY) +0.1
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
b4151 (frdD) +0.3
b4152 (frdC) +0.2
b4153 (frdB) -0.0
b4154 (frdA) +0.1
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
b0118 (acnB) -0.2
b0771 (ybhJ) -0.1
b1276 (acnA) -0.7
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
b1136 (icd) N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information