MetaCyc Pathway: L-citrulline biosynthesis in Escherichia coli BW25113

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Reactions and Genes Ying_AminoAcid20 rep A; time point 3
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Arginase:
L-arginine + H2O→L-ornithine + urea (EC 3.5.3.1)
No genes
Glutamate synthase (NADPH):
L-glutamine + H2O→L-glutamate + ammonia + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
b0032 (carA) -5.6
b0033 (carB) -7.4
b0485 (ybaS) +0.2
b0674 (asnB) -0.1
b1263 (trpD) -0.3
b1264 (trpE) -0.2
b1524 (yneH) +0.1
b1812 (pabB) -0.1
b2468 (aegA) -0.0
b2507 (guaA) N.D.
b2611 (ypjD) -0.0
b2780 (pyrG) N.D.
b2887 (ygfT) +0.2
b3212 (gltB) -0.0
b3213 (gltD) -0.0
b3360 (pabA) -0.3
L-proline[in] + an electron-transfer quinone→(S)-1-pyrroline-5-carboxylate[in] + an electron-transfer quinol + H+[in] (EC 1.5.5.2)
b1014 (putA) -1.3
Glutamate 5-kinase:
L-glutamate + ATP→γ-L-glutamyl 5-phosphate + ADP (EC 2.7.2.11)
b0242 (proB) -0.3
(S)-1-pyrroline-5-carboxylate + H+ + H2O→L-glutamate 5-semialdehyde (spontaneous)
No genes
Glutamate-5-semialdehyde dehydrogenase (in reverse):
γ-L-glutamyl 5-phosphate + NADPH + H+→L-glutamate 5-semialdehyde + NADP+ + phosphate (EC 1.2.1.41)
b0243 (proA) -0.2
Ornithine aminotransferase (in reverse):
L-glutamate + L-glutamate 5-semialdehyde→L-ornithine + 2-oxoglutarate (EC 2.6.1.13)
No genes
Ornithine carbamoyltransferase:
L-ornithine + carbamoyl phosphate→L-citrulline + H+ + phosphate (EC 2.1.3.3)
b0273 (argF) -0.0
b4254 (argI) +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information