MetaCyc Pathway: C4 photosynthetic carbon assimilation cycle, PEPCK type in Escherichia coli BW25113

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Reactions and Genes Ying_all64 rep A; time point 3
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Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+
(EC 4.2.1.1)
b0126 (can) N.D.
b0339 (cynT) +0.1
Phosphoenolpyruvate carboxylase (in reverse):
hydrogen carbonate + phosphoenolpyruvate→oxaloacetate + phosphate
(EC 4.1.1.31)
b3956 (ppc) -0.4
Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate
(EC 2.6.1.1)
b0928 (aspC) -1.0
b4340 (yjiR) +0.1
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) (in reverse):
oxaloacetate + NADPH + H+→(S)-malate + NADP+
(EC 1.1.1.40; 1.1.1.82)
b2463 (maeB) +0.1
L-aspartate[in]→L-aspartate[out]
No genes
(S)-malate[in]→(S)-malate[out]
No genes
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)):
(S)-malate + NADP+→CO2 + pyruvate + NADPH
(EC 1.1.1.40)
b2463 (maeB) +0.1
Aspartate transaminase:
L-aspartate + 2-oxoglutarate→oxaloacetate + L-glutamate
(EC 2.6.1.1)
b0928 (aspC) -1.0
b4340 (yjiR) +0.1
Alanine transaminase (in reverse):
pyruvate + L-glutamate→L-alanine + 2-oxoglutarate
(EC 2.6.1.2)
b2290 (yfbQ) -1.7
b2379 (yfdZ) -0.6
Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→CO2 + phosphoenolpyruvate + ADP
(EC 4.1.1.49)
b3403 (pck) +0.1
L-alanine[in]→L-alanine[out]
No genes
phosphoenolpyruvate[in]→phosphoenolpyruvate[out]
No genes
Alanine transaminase (in reverse):
L-alanine + 2-oxoglutarate→pyruvate + L-glutamate
(EC 2.6.1.2)
b2290 (yfbQ) -1.7
Pyruvate, phosphate dikinase:
pyruvate + ATP + phosphate→phosphoenolpyruvate + AMP + diphosphate + H+
(EC 2.7.9.1)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information