MetaCyc Pathway: γ-glutamyl cycle in Escherichia coli BW25113

Reactions and Genes	Ying_Nucleobase14 rep A; time point 2
5-oxoprolinase (ATP-hydrolyzing): 5-oxo-L-proline + ATP + 2 H2O→L-glutamate + ADP + H+ + phosphate (EC 3.5.2.9)
b0711 (ybgJ)	+0.1
b0712 (ybgK)	+0.1
b0713 (ybgL)	-0.1
Glutamate--cysteine ligase: L-glutamate + L-cysteine + ATP→γ-L-glutamyl-L-cysteine + ADP + H+ + phosphate (EC 6.3.2.2)
b0581 (ybdK)	-0.2
b2688 (gshA)	+3.2
Glutathione synthase: γ-L-glutamyl-L-cysteine + ATP + glycine→glutathione + ADP + H+ + phosphate (EC 6.3.2.3)
b2947 (gshB)	+2.8
Gamma-glutamyltransferase: glutathione + a proteinogenic amino acid→an α-(γ-L-glutamyl)-L-amino acid + L-cysteinylglycine (EC 2.3.2.2)
b3447 (ggt)	-0.1
an α-(γ-L-glutamyl)-L-amino acid→5-oxo-L-proline + an L-amino acid (EC 4.3.2.9)
No genes
Cytosol nonspecific dipeptidase: L-cysteinylglycine + H2O→L-cysteine + glycine (EC 3.4.13.18)
b0237 (pepD)	-0.2
b0932 (pepN)	-0.0
b2385 (ypdF)	-0.0
b2523 (pepB)	+0.3
b4260 (pepA)	+1.8

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information