MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Escherichia coli BW25113

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Reactions and Genes Ying_Nucleobase14 rep A; time point 2
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Mannitol-1-phosphate 5-dehydrogenase:
D-mannitol 1-phosphate + NAD+→β-D-fructofuranose 6-phosphate + NADH + H+ (EC 1.1.1.17)
b3600 (mtlD) -0.2
Sorbitol-6-phosphate 2-dehydrogenase:
D-sorbitol 6-phosphate + NAD+→keto-D-fructose 6-phosphate + NADH + H+ (EC 1.1.1.140)
b2705 (srlD) -0.1
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
b1723 (pfkB) +0.1
b3916 (pfkA) -3.1
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
b2097 (b2097) +0.3
b2925 (fbaA) N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
b0383 (phoA) +0.1
b0766 (ybhA) -0.1
b2930 (yggF) +0.1
b3925 (glpX) -0.1
b4232 (fbp) +0.3
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
b3919 (tpiA) N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
b1779 (gapA) N.D.
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
b2926 (pgk) N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
b0755 (gpmA) -1.0
b3612 (gpmI) +0.0
b4395 (ytjC) -1.4
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
b0755 (gpmA) -1.0
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
b2779 (eno) N.D.
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
b1702 (pps) -0.1
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF) -1.6
b1854 (pykA) -0.3
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
b0823 (ybiW) +0.1
b0824 (ybiY) -0.0
b0902 (pflA) -0.1
b0903 (pflB) -0.1
b2579 (yfiD) -0.1
b3951 (pflD) -0.0
b3952 (pflC) -0.1
b3114 (tdcE) -0.0
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
b0351 (mhpF) -0.1
b1241 (adhE) +0.2
b2455 (eutE) -0.0
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
b0306 (ykgE) +0.1
b0307 (ykgF) -0.1
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
b2297 (pta) -0.5
b2458 (eutI) -0.1
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT) -0.1
b2296 (ackA) N.D.
b2460 (eutQ) +0.0
b2461 (eutP) +0.0
b3115 (tdcD) +0.1
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
b0325 (yahK) +0.0
b0356 (frmA) -0.0
b1241 (adhE) +0.2
b1478 (adhP) -0.3
b3253 (yhdH) +0.1
b4269 (yjgB) +0.0
b3589 (yiaY) +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information