MetaCyc Pathway: TCA cycle IV (2-oxoglutarate decarboxylase) in Escherichia coli BW25113

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Reactions and Genes Ying_AminoAcid20 rep A; time point 2
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Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
b0118 (acnB) -0.3
b0771 (ybhJ) -0.1
b1276 (acnA) -0.4
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
b0118 (acnB) -0.3
b0771 (ybhJ) -0.1
b1276 (acnA) -0.4
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
b1136 (icd) N.D.
2-oxoglutarate decarboxylase:
2-oxoglutarate + H+→succinate semialdehyde + CO2 (EC 4.1.1.71)
No genes
succinate semialdehyde + NADP+ + H2O→succinate + NADPH + 2 H+ (EC 1.2.1.79)
b2661 (gabD) -0.0
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
No genes
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
b1611 (fumC) -1.3
b1612 (fumA) -0.2
b1675 (ydhZ) -0.4
b2929 (yggD) +0.2
b4122 (fumB) -0.0
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC) +0.1
b1479 (sfcA) +0.1
b3236 (mdh) -4.5
Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
b0720 (gltA) -1.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information