MetaCyc Pathway: L-arginine biosynthesis III (via N-acetyl-L-citrulline) in Escherichia coli BW25113

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Reactions and Genes M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
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Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
hydrogen carbonate + 2 ATP + L-glutamine + H2O→carbamoyl phosphate + L-glutamate + 2 ADP + 2 H+ + phosphate (EC 6.3.5.5)
b0032 (carA) -2.0
b0033 (carB) -1.9
Amino-acid N-acetyltransferase:
L-glutamate + acetyl-CoA→N-acetyl-L-glutamate + coenzyme A + H+ (EC 2.3.1.1)
b2818 (argA) -1.9
Acetylglutamate kinase:
N-acetyl-L-glutamate + ATP→N-acetylglutamyl-phosphate + ADP (EC 2.7.2.8)
b3959 (argB) -1.6
N-acetyl-gamma-glutamyl-phosphate reductase (in reverse):
N-acetylglutamyl-phosphate + NADPH + H+→N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate (EC 1.2.1.38)
b3958 (argC) -1.7
Acetylornithine transaminase (in reverse):
N-acetyl-L-glutamate 5-semialdehyde + L-glutamate→N-acetyl-L-ornithine + 2-oxoglutarate (EC 2.6.1.11)
b1748 (astC) -0.0
b2662 (gabT) +0.2
b3359 (argD) -1.3
N-acetylornithine carbamoyltransferase:
carbamoyl phosphate + N-acetyl-L-ornithine→N-acetyl-L-citrulline + H+ + phosphate (EC 2.1.3.9)
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Acetylornithine deacetylase:
N-acetyl-L-citrulline + H2O→L-citrulline + acetate (EC 3.5.1.16)
b3957 (argE) -1.7
Argininosuccinate synthase:
L-citrulline + ATP + L-aspartate→L-arginino-succinate + AMP + diphosphate + H+ (EC 6.3.4.5)
b3172 (argG) -3.8
Argininosuccinate lyase:
L-arginino-succinate→L-arginine + fumarate (EC 4.3.2.1)
b3960 (argH) -4.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information