MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Pseudomonas putida KT2440

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Reactions and Genes Ammonium chloride and TNT (N)
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
PP_0269 -0.2
PP_0417 -4.2
PP_0420 -1.4
PP_0511 -0.1
PP_0613 -0.0
PP_0727 -0.2
PP_0930 N.D.
PP_0931 N.D.
PP_0932 N.D.
PP_0991 -0.0
PP_1032 N.D.
PP_1060 +0.2
PP_1374 -0.5
PP_1460 -1.2
PP_1610 N.D.
PP_1691 -0.8
PP_1750 +0.1
PP_1842 +0.2
PP_2323 +0.3
PP_2329 +0.3
PP_2447 -0.0
PP_2453 -0.2
PP_3464 +0.2
PP_3598 +0.1
PP_4037 +0.2
PP_4723 -3.3
PP_4724 -2.5
PP_5075 -1.6
PP_5076 -1.4
Aspartate--tRNA(Asn) ligase:
L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate (EC 6.1.1.23)
PP_1213 N.D.
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP (EC 6.3.5.6)
PP_0613 -0.0
PP_0930 N.D.
PP_0931 N.D.
PP_0932 N.D.
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate (EC 6.3.5.6)
PP_0613 -0.0
PP_0930 N.D.
PP_0931 N.D.
PP_0932 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information