MetaCyc Pathway: (2S)-ethylmalonyl-CoA biosynthesis in Escherichia coli BW25113
| Reactions and Genes | vcDART vector transformation; liquid outgrowth assay |
|---|---|
| remove | |
| Acetyl-CoA C-acyltransferase: 2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9) | |
| b1397 (paaJ) | -0.1 |
| b2224 (atoB) | +0.0 |
| b2342 (yfcY) | -0.4 |
| b2844 (yqeF) | -2.0 |
| b3845 (fadA) | +0.0 |
| Acetoacetyl-CoA reductase (in reverse): acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36) | |
| No genes | |
| 3-hydroxybutyryl-CoA dehydratase: (3R)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.55) | |
| b1387 (maoC) | -0.1 |
| crotonyl-CoA + CO2 + NADPH→(2S)-ethylmalonyl-CoA + NADP+ (EC 1.3.1.85) | |
| No genes | |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information