MetaCyc Pathway: incomplete reductive TCA cycle in Escherichia coli BW25113

Reactions and Genes	vcDART vector transformation; agar assay
Pyruvate synthase (in reverse): acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
b1378 (ydbK)	+0.3
Pyruvate carboxylase: pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Malate dehydrogenase (in reverse): oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC)	-0.2
b1479 (sfcA)	+0.0
b3236 (mdh)	+1.7
Fumarate hydratase: (S)-malate→fumarate + H2O (EC 4.2.1.2)
b1611 (fumC)	+0.2
b1612 (fumA)	+2.1
b1675 (ydhZ)	+0.2
b2929 (yggD)	+0.1
b4122 (fumB)	-0.0
Succinate dehydrogenase (ubiquinone): fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
b4151 (frdD)	+0.1
b4152 (frdC)	-0.1
b4153 (frdB)	+0.4
b4154 (frdA)	+0.1
Succinate--CoA ligase (ADP-forming): succinate + ATP + coenzyme A→succinyl-CoA + ADP + phosphate (EC 6.2.1.5)
b0728 (sucC)	+0.9
b0729 (sucD)	+0.9
2-oxoglutarate synthase (in reverse): succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information