MetaCyc Pathway: superpathway of L-tyrosine biosynthesis in Escherichia coli BW25113

Reactions and Genes	M9 with Methotrexate 0.1 mM 48 hr
3-deoxy-7-phosphoheptulonate synthase: D-erythrose 4-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (EC 2.5.1.54)
b0754 (aroG)	+1.0
b1704 (aroH)	-0.1
b2601 (aroF)	-0.7
3-dehydroquinate synthase: 3-deoxy-D-arabino-heptulosonate 7-phosphate→3-dehydroquinate + phosphate (EC 4.2.3.4)
b3389 (aroB)	-2.8
3-dehydroquinate dehydratase: 3-dehydroquinate→3-dehydroshikimate + H2O (EC 4.2.1.10)
b1693 (aroD)	-2.3
b2779 (eno)	N.D.
Shikimate dehydrogenase (in reverse): 3-dehydroshikimate + NADPH + H+→shikimate + NADP+ (EC 1.1.1.25)
b1692 (ydiB)	+0.2
b3281 (aroE)	-2.7
Shikimate kinase: shikimate + ATP→shikimate 3-phosphate + ADP + H+ (EC 2.7.1.71)
b0388 (aroL)	+0.3
b3390 (aroK)	-1.4
3-phosphoshikimate 1-carboxyvinyltransferase: shikimate 3-phosphate + phosphoenolpyruvate→5-enolpyruvoyl-shikimate 3-phosphate + phosphate (EC 2.5.1.19)
b0908 (aroA)	-2.7
Chorismate synthase: 5-enolpyruvoyl-shikimate 3-phosphate→chorismate + phosphate (EC 4.2.3.5)
b2329 (aroC)	-2.6
Chorismate mutase: chorismate→prephenate (EC 5.4.99.5)
b2599 (pheA)	-0.4
b2600 (tyrA)	-2.0
Prephenate dehydrogenase: prephenate + NAD+→3-(4-hydroxyphenyl)pyruvate + CO2 + NADH (EC 1.3.1.12)
b2600 (tyrA)	-2.0
Aspartate transaminase (in reverse): 3-(4-hydroxyphenyl)pyruvate + L-glutamate→L-tyrosine + 2-oxoglutarate (EC 2.6.1.1; 2.6.1.27; 2.6.1.5; 2.6.1.57)
b0928 (aspC)	-1.0
b2021 (hisC)	-1.8
b4054 (tyrB)	-0.3
b4340 (yjiR)	-0.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information