MetaCyc Pathway: chorismate biosynthesis II (archaea) in Escherichia coli BW25113

Reactions and Genes	M9 with Methotrexate 0.06 mM 48 hr
Aspartate kinase: L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
b0002 (thrA)	-2.5
b3940 (metL)	-0.5
b4024 (lysC)	-0.4
D-glyceraldehyde 3-phosphate→enolaldehyde + phosphate
No genes
Aspartate-semialdehyde dehydrogenase (in reverse): L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
b2319 (usg)	+0.1
b3433 (asd)	N.D.
enolaldehyde→methylglyoxal (spontaneous)
No genes
methylglyoxal + β-D-fructofuranose 1,6-bisphosphate→D-glyceraldehyde 3-phosphate + 6-deoxy-5-ketofructose 1-phosphate (EC 2.2.1.11)
No genes
6-deoxy-5-ketofructose 1-phosphate + L-aspartate 4-semialdehyde→2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + hydroxypyruvaldehyde phosphate (EC 2.2.1.10)
No genes
2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate + NAD+ + H2O→3-dehydroquinate + ammonium + NADH + H+ (EC 1.4.1.24)
No genes
3-dehydroquinate dehydratase: 3-dehydroquinate→3-dehydroshikimate + H2O (EC 4.2.1.10)
b1693 (aroD)	-2.2
b2779 (eno)	N.D.
Shikimate dehydrogenase (in reverse): 3-dehydroshikimate + NADPH + H+→shikimate + NADP+ (EC 1.1.1.25)
b1692 (ydiB)	+0.0
b3281 (aroE)	-3.1
Shikimate kinase: shikimate + ATP→shikimate 3-phosphate + ADP + H+ (EC 2.7.1.71)
b0388 (aroL)	+1.4
b3390 (aroK)	-1.1
3-phosphoshikimate 1-carboxyvinyltransferase: shikimate 3-phosphate + phosphoenolpyruvate→5-enolpyruvoyl-shikimate 3-phosphate + phosphate (EC 2.5.1.19)
b0908 (aroA)	-2.4
Chorismate synthase: 5-enolpyruvoyl-shikimate 3-phosphate→chorismate + phosphate (EC 4.2.3.5)
b2329 (aroC)	-2.9

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information