MetaCyc Pathway: chorismate biosynthesis I in Escherichia coli BW25113

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Reactions and Genes M9 with Methotrexate 0.06 mM 48 hr
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3-deoxy-7-phosphoheptulonate synthase:
D-erythrose 4-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (EC 2.5.1.54)
b0754 (aroG) +2.1
b1704 (aroH) -0.1
b2601 (aroF) -1.0
3-dehydroquinate synthase:
3-deoxy-D-arabino-heptulosonate 7-phosphate→3-dehydroquinate + phosphate (EC 4.2.3.4)
b3389 (aroB) -2.6
3-dehydroquinate dehydratase:
3-dehydroquinate→3-dehydroshikimate + H2O (EC 4.2.1.10)
b1693 (aroD) -2.2
b2779 (eno) N.D.
Shikimate dehydrogenase (in reverse):
3-dehydroshikimate + NADPH + H+→shikimate + NADP+ (EC 1.1.1.25)
b1692 (ydiB) +0.0
b3281 (aroE) -3.1
Shikimate kinase:
shikimate + ATP→shikimate 3-phosphate + ADP + H+ (EC 2.7.1.71)
b0388 (aroL) +1.4
b3390 (aroK) -1.1
3-phosphoshikimate 1-carboxyvinyltransferase:
shikimate 3-phosphate + phosphoenolpyruvate→5-enolpyruvoyl-shikimate 3-phosphate + phosphate (EC 2.5.1.19)
b0908 (aroA) -2.4
Chorismate synthase:
5-enolpyruvoyl-shikimate 3-phosphate→chorismate + phosphate (EC 4.2.3.5)
b2329 (aroC) -2.9

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information