MetaCyc Pathway: reductive TCA cycle I in Escherichia coli BW25113

Add experiment(s):


Reactions and Genes M9 no stress control; Anaerobic 24 hr
remove
2-oxoglutarate synthase (in reverse):
succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
No genes
Isocitrate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + CO2 + NADPH→D-threo-isocitrate + NADP+ (EC 1.1.1.42)
b1136 (icd) N.D.
Aconitate hydratase (in reverse):
D-threo-isocitrate→cis-aconitate + H2O (EC 4.2.1.3)
b0118 (acnB) -0.0
b0771 (ybhJ) -0.0
b1276 (acnA) -0.2
Aconitate hydratase (in reverse):
cis-aconitate + H2O→citrate (EC 4.2.1.3)
b0118 (acnB) -0.0
b0771 (ybhJ) -0.0
b1276 (acnA) -0.2
ATP citrate synthase (in reverse):
citrate + ATP + coenzyme A→acetyl-CoA + oxaloacetate + ADP + phosphate (EC 2.3.3.8)
No genes
Pyruvate synthase (in reverse):
acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
b1378 (ydbK) -0.3
Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC) +0.1
b1479 (sfcA) -0.1
b3236 (mdh) +1.7
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
b1611 (fumC) +0.2
b1612 (fumA) -0.3
b1675 (ydhZ) +0.0
b2929 (yggD) +0.3
b4122 (fumB) +0.8
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
b4151 (frdD) +1.7
b4152 (frdC) +1.7
b4153 (frdB) +1.5
b4154 (frdA) +1.6
Succinate--CoA ligase (ADP-forming):
succinate + ATP + coenzyme A→succinyl-CoA + ADP + phosphate (EC 6.2.1.5)
b0728 (sucC) -2.1
b0729 (sucD) -3.1

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information