MetaCyc Pathway: superpathway of L-threonine biosynthesis in Escherichia coli BW25113

Add experiment(s):


Reactions and Genes M9 no stress control; Anaerobic 24 hr
remove
Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
b0928 (aspC) -5.4
b4340 (yjiR) +0.0
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
b0002 (thrA) -5.1
b3940 (metL) -0.4
b4024 (lysC) -1.1
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
b2319 (usg) -0.2
b3433 (asd) N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
b0002 (thrA) -5.1
b3940 (metL) -0.4
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
b0003 (thrB) -5.0
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
b0004 (thrC) -5.3

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information