MetaCyc Pathway: superpathway of photosynthetic hydrogen production in Escherichia coli BW25113

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Reactions and Genes M9 with Methotrexate 0.1 mM 48 hr
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2 H2O[in] + hν[cytosol] + 2 a plastoquinone + 4 H+[out]→2 a plastoquinol + dioxygen[in] + 4 H+[in] (EC 1.10.3.9)
No genes
NAD(P)H dehydrogenase (quinone):
NAD(P)H + a plastoquinone + H+→a plastoquinol + NAD(P)+ (EC 1.6.5.2)
b1004 (wrbA) +0.1
a plastoquinol + 2 an oxidized plastocyanin[in] + 2 H+[out]→2 a reduced plastocyanin[in] + a plastoquinone + 4 H+[in] (EC 7.1.1.6)
No genes
a reduced plastocyanin[in] + hν[cytosol] + an oxidized ferredoxin [iron-sulfur] cluster[out]→a reduced ferredoxin [iron-sulfur] cluster[out] + an oxidized plastocyanin[in] (EC 1.97.1.12)
No genes
Ferredoxin--NADP(+) reductase:
2 a reduced ferredoxin [iron-sulfur] cluster[out] + NADP+[out] + H+[out]→NADPH[out] + 2 an oxidized ferredoxin [iron-sulfur] cluster[out] (EC 1.18.1.2)
b3924 (fpr) -1.2
Ferredoxin hydrogenase (in reverse):
2 a reduced ferredoxin [iron-sulfur] cluster + 2 H+→H2 + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.12.7.2)
b2483 (hyfC) +0.0
b2719 (hycG) +0.2
b2720 (hycF) -0.2
b2721 (hycE) -0.0
b2722 (hycD) -0.2
b2723 (hycC) +0.1
b2724 (hycB) -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information