MetaCyc Pathway: butanol and isobutanol biosynthesis (engineered) in Pseudomonas sp. RS175

Reactions and Genes	L-Hydroxyproline (C) 10 mM
	remove
Glycine oxidase: glycine + dioxygen + H₂O→glyoxylate + ammonium + hydrogen peroxide (EC 1.4.3.19; 1.4.3.3)
PFR28_01786	-3.9
PFR28_03753	-0.0
3-ethylmalate synthase: glyoxylate + butanoyl-CoA + H₂O→3-ethylmalate + coenzyme A + H⁺ (EC 2.3.3.7)
No genes
3-isopropylmalate dehydrogenase: 3-ethylmalate + NAD⁺→2-oxovalerate + CO₂ + NADH (EC 1.1.1.85)
PFR28_02018	+0.0
PFR28_02821	-3.8
2-oxovalerate→3-methyl-2-oxobutanoate
No genes
Pyruvate decarboxylase: 2-oxovalerate + H⁺→1-butanal + CO₂ (EC 4.1.1.1)
No genes
1-butanal + NADH + H⁺→butan-1-ol + NAD⁺
No genes
Branched-chain-2-oxoacid decarboxylase: 3-methyl-2-oxobutanoate + H⁺→isobutanal + CO₂ (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse): isobutanal + NADH + H⁺→isobutanol + NAD⁺ (EC 1.1.1.1)
PFR28_00420	+0.3
PFR28_00654	+0.0
PFR28_01820	-0.1
PFR28_01972	+0.2
PFR28_02982	+0.1
PFR28_04050	+0.0

Links:

Pathway details at MetaCyc
Fitness data for 10 genes
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All MetaCyc pathways for Pseudomonas sp. RS175

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information