MetaCyc Pathway: superpathway of fatty acids biosynthesis (E. coli) in Pseudomonas simiae WCS417

Add experiment(s):


Reactions and Genes Doxycycline hyclate 0.001 mg/ml
remove
acetyl-CoA + a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
PS417_06290 N.D.
PS417_18555 N.D.
[Acyl-carrier-protein] S-malonyltransferase:
malonyl-CoA + a soluble [acyl-carrier protein]→a malonyl-[acp] + coenzyme A (EC 2.3.1.39)
PS417_21445 N.D.
PS417_26590 +0.1
Beta-ketoacyl-acyl-carrier-protein synthase III:
acetyl-CoA + a malonyl-[acp] + H+→an acetoacetyl-[acp] + CO2 + coenzyme A (EC 2.3.1.180)
PS417_19855 N.D.
PS417_20570 -0.7
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an acetoacetyl-[acp] + NADPH + H+→a (3R)-3-hydroxybutanoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxybutanoyl-[acp]→a (2E)-but-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-but-2-enoyl-[acp] + NADH + H+→a butanoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a butanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxohexanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PS417_08975 N.D.
PS417_13680 +0.2
PS417_02030 +0.3
PS417_02045 +0.4
PS417_21430 +1.8
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxohexanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyhexanoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyhexanoyl-[acp]→a (2E)-hex-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-hex-2-enoyl-[acp] + NADH + H+→a hexanoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a hexanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PS417_08975 N.D.
PS417_13680 +0.2
PS417_02030 +0.3
PS417_02045 +0.4
PS417_21430 +1.8
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxooctanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyoctanoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyoctanoyl-[acp]→a (2E)-oct-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-oct-2-enoyl-[acp] + NADH + H+→an octanoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
an octanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PS417_08975 N.D.
PS417_13680 +0.2
PS417_02030 +0.3
PS417_02045 +0.4
PS417_21430 +1.8
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxodecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxydecanoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dec-2-enoyl-[acp]→a (3Z)-dec-3-enoyl-[acp] (EC 5.3.3.14)
PS417_08980 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-dec-2-enoyl-[acp] + NADH + H+→a decanoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a decanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PS417_08975 N.D.
PS417_13680 +0.2
PS417_02030 +0.3
PS417_02045 +0.4
PS417_21430 +1.8
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxododec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PS417_08975 N.D.
PS417_13680 +0.2
PS417_02030 +0.3
PS417_02045 +0.4
PS417_21430 +1.8
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxododecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxydodecanoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxododec-5-enoyl-[acp] + NADPH + H+→a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydodecanoyl-[acp]→a (2E)-dodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp]→a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-dodec-2-enoyl-[acp] + NADH + H+→a dodecanoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + NADH + H+→a (5Z)-dodec-5-enoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Dodecanoyl-[acyl-carrier-protein] hydrolase:
a dodecanoyl-[acp][nil] + H2O[nil]→laurate[nil] + a soluble [acyl-carrier protein][nil] + H+[nil] (EC 3.1.2.21)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (5Z)-dodec-5-enoyl-[acp] + a malonyl-[acp] + H+→a (7Z)-3-oxotetradec-7-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PS417_08975 N.D.
PS417_13680 +0.2
PS417_02030 +0.3
PS417_02045 +0.4
PS417_21430 +1.8
Beta-ketoacyl-acyl-carrier-protein synthase II:
a dodecanoyl-[acp] + a malonyl-[acp] + H+→a 3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PS417_08975 N.D.
PS417_13680 +0.2
PS417_02030 +0.3
PS417_02045 +0.4
PS417_21430 +1.8
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (7Z)-3-oxotetradec-7-enoyl-[acp] + NADPH + H+→a (3R,7Z)-3-hydroxytetradec-7-enoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
Butyrate--CoA ligase:
laurate + ATP + coenzyme A→lauroyl-CoA + AMP + diphosphate (EC 6.2.1.2; 6.2.1.3)
PS417_15170 +0.1
PS417_17010 -0.3
PS417_19290 +0.1
PS417_22065 +1.5
PS417_22070 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxytetradecanoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,7Z)-3-hydroxytetradec-7-enoyl-[acp]→a (2E,7Z)-tetradeca-2,7-dienoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxytetradecanoyl-[acp]→a (2E)-tetradec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,7Z)-tetradeca-2,7-dienoyl-[acp] + NADH + H+→a (7Z)-tetradec-7-enoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-tetradec-2-enoyl-[acp] + NADH + H+→a myristoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (7Z)-tetradec-7-enoyl-[acp] + a malonyl-[acp] + H+→a (9Z)-3-oxohexadec-9-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PS417_08975 N.D.
PS417_13680 +0.2
PS417_02030 +0.3
PS417_02045 +0.4
PS417_21430 +1.8
Beta-ketoacyl-acyl-carrier-protein synthase II:
a myristoyl-[acp] + a malonyl-[acp] + H+→a 3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PS417_08975 N.D.
PS417_13680 +0.2
PS417_02030 +0.3
PS417_02045 +0.4
PS417_21430 +1.8
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (9Z)-3-oxohexadec-9-enoyl-[acp] + NADPH + H+→a (3R,9Z)-3-hydroxyhexadec-9-enoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxohexadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxyhexadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyhexadecanoyl-[acp]→a (2E)-hexadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,9Z)-3-hydroxyhexadec-9-enoyl-[acp]→a (2E,9Z)-hexadeca-2,9-dienoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,9Z)-hexadeca-2,9-dienoyl-[acp] + NADH + H+→a palmitoleoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-hexadec-2-enoyl-[acp] + NADH + H+→a palmitoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Oleoyl-[acyl-carrier-protein] hydrolase:
a palmitoyl-[acp] + H2O→palmitate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
Oleoyl-[acyl-carrier-protein] hydrolase:
a palmitoleoyl-[acp] + H2O→palmitoleate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a palmitoleoyl-[acp] + a malonyl-[acp] + H+→an (11Z)-3-oxooctadec-11-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
PS417_13680 +0.2
PS417_02030 +0.3
PS417_21430 +1.8
Long-chain-fatty-acid--CoA ligase:
palmitate + ATP + coenzyme A→palmitoyl-CoA + AMP + diphosphate (EC 6.2.1.3)
PS417_15170 +0.1
PS417_17010 -0.3
PS417_19290 +0.1
PS417_22065 +1.5
PS417_22070 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an (11Z)-3-oxooctadec-11-enoyl-[acp] + NADPH + H+→a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp] + NADP+ (EC 1.1.1.100)
PS417_05265 N.D.
PS417_05270 +0.2
PS417_11810 +0.5
PS417_01365 N.D.
PS417_13755 -0.7
PS417_15185 -0.1
PS417_16870 -0.5
PS417_17710 N.D.
PS417_17895 N.D.
PS417_02035 +0.6
PS417_21145 +0.5
PS417_21340 -0.5
PS417_21440 N.D.
PS417_03140 -0.2
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,11Z)-3-hydroxyoctadec-11-enoyl-[acp]→a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + H2O (EC 4.2.1.59)
PS417_06265 N.D.
PS417_08980 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,11Z)-octadeca-2,11-dienoyl-[acp] + NADH + H+→a cis-vaccenoyl-[acp] + NAD+ (EC 1.3.1.9)
PS417_14110 -0.0
Oleoyl-[acyl-carrier-protein] hydrolase:
a cis-vaccenoyl-[acp] + H2O→cis-vaccenate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information