MetaCyc Pathway: cutin biosynthesis in Pseudomonas simiae WCS417

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Reactions and Genes Doxycycline hyclate 0.001 mg/ml
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Palmitoyl-CoA hydrolase:
palmitoyl-CoA + H2O→palmitate + coenzyme A + H+
(EC 3.1.2.2)
No genes
Palmitoyl-CoA hydrolase:
oleoyl-CoA + H2O→oleate + coenzyme A + H+
(EC 3.1.2.2)
No genes
palmitate + dioxygen + a reduced [NADPH-hemoprotein reductase]→16-hydroxypalmitate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.80)
No genes
oleate + dioxygen + a reduced [NADPH-hemoprotein reductase]→18-hydroxyoleate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.80)
No genes
oleate + a hydroperoxy-fatty-acyl-[lipid]→9,10-epoxystearate + a hydroxy-fatty-acyl-[lipid] (EC 1.11.2.3)
No genes
18-hydroxyoleate + a hydroperoxy-fatty-acyl-[lipid]→9,10-epoxy-18-hydroxystearate + a hydroxy-fatty-acyl-[lipid] (EC 1.11.2.3)
No genes
Long-chain-fatty-acid--CoA ligase:
16-hydroxypalmitate + ATP + coenzyme A→16-hydroxypalmitoyl-CoA + AMP + diphosphate
(EC 6.2.1.3)
PS417_15170 +0.1
PS417_17010 -0.3
PS417_19290 +0.1
PS417_22065 +1.5
PS417_22070 -0.1
18-hydroxyoleate + NADP+→18-oxo-oleate + NADPH + H+
No genes
16-hydroxypalmitate + NADP+→16-oxo-palmitate + NADPH + H+
No genes
9,10-epoxystearate + dioxygen + a reduced [NADPH-hemoprotein reductase]→9,10-epoxy-18-hydroxystearate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.80)
No genes
9,10-epoxystearate + H2O→(9R,10S)-dihydroxystearate
No genes
16-hydroxypalmitoyl-CoA + NADPH + dioxygen + H+→10,16-dihydroxypalmitoyl-CoA + NADP+ + H2O
No genes
9,10-epoxy-18-hydroxystearate + H2O→9,10,18-trihydroxystearate
No genes
18-oxo-oleate + NADP+ + H2O→α,ω-9Z-octadecenedioate + NADPH + 2 H+
No genes
16-oxo-palmitate + NADP+ + H2O→hexadecanedioate + NADPH + 2 H+
No genes
(9R,10S)-dihydroxystearate + dioxygen + a reduced [NADPH-hemoprotein reductase]→9,10,18-trihydroxystearate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.80)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information