MetaCyc Pathway: superpathway of L-lysine, L-threonine and L-methionine biosynthesis II in Pseudomonas fluorescens SBW25

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Reactions and Genes NAG (C)
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Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP
(EC 2.7.2.4)
PFLU_RS23265 -0.1
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate
(EC 1.2.1.11)
PFLU_RS06680 -0.4
PFLU_RS20555 -0.2
PFLU_RS20560 N.D.
L-aspartate 4-semialdehyde + pyruvate→(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H+ + H2O (EC 4.3.3.7)
PFLU_RS06945 -0.0
PFLU_RS07275 -1.9
PFLU_RS09565 -0.1
PFLU_RS16615 -0.1
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+
(EC 1.1.1.3)
PFLU_RS09890 +0.1
PFLU_RS24550 -3.9
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+
(EC 2.7.1.39)
PFLU_RS00385 -0.2
PFLU_RS22735 -1.5
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+→(S)-2,3,4,5-tetrahydrodipicolinate + NAD(P)+ + H2O (EC 1.17.1.8)
PFLU_RS25890 N.D.
Cystathionine gamma-synthase:
O-phospho-L-homoserine + L-cysteine→L-cystathionine + phosphate
(EC 2.5.1.48)
PFLU_RS02250 +0.0
PFLU_RS16625 -0.1
PFLU_RS20510 -3.7
LL-diaminopimelate aminotransferase (in reverse):
(S)-2,3,4,5-tetrahydrodipicolinate + L-glutamate + H+ + H2O→L,L-diaminopimelate + 2-oxoglutarate
(EC 2.6.1.83)
No genes
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate
(EC 4.2.3.1)
PFLU_RS09885 -0.7
PFLU_RS24545 -3.9
Diaminopimelate epimerase:
L,L-diaminopimelate→meso-diaminopimelate
(EC 5.1.1.7)
PFLU_RS10355 -0.3
PFLU_RS13140 +0.1
PFLU_RS17335 -0.1
PFLU_RS23725 -3.9
PFLU_RS29270 -1.5
Cysteine-S-conjugate beta-lyase:
L-cystathionine→2-aminoprop-2-enoate + L-homocysteine
(EC 4.4.1.13)
No genes
Diaminopimelate decarboxylase:
meso-diaminopimelate + H+→L-lysine + CO2
(EC 4.1.1.20)
PFLU_RS29265 -3.7
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate
(EC 2.1.1.14)
PFLU_RS17405 +0.0
Tryptophanase:
2-aminoprop-2-enoate→2-iminopropanoate
(spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
PFLU_RS01220 -0.6
PFLU_RS05135 +0.0
PFLU_RS12815 -1.7
PFLU_RS16625 -0.1
PFLU_RS24005 +0.1
PFLU_RS27960 -0.1
2-iminopropanoate + H2O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
PFLU_RS01220 -0.6
PFLU_RS05135 +0.0
PFLU_RS12815 -1.7
PFLU_RS16625 -0.1
PFLU_RS24005 +0.1
PFLU_RS27960 -0.1
PFLU_RS29515 -0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information