MetaCyc Pathway: Rubisco shunt in Escherichia coli BW25113

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Reactions and Genes 2,4-Dinitrophenol 2 mM
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Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-erythrose 4-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
b2465 (tktB) -0.3
b2935 (tktA) -3.5
Transaldolase (in reverse):
D-erythrose 4-phosphate + β-D-fructofuranose 6-phosphate→D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate (EC 2.2.1.2)
b0008 (talB) -1.3
b0825 (mipB) +0.1
b2464 (talA) -0.4
b3946 (fsaB) -0.1
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-ribose 5-phosphate (EC 2.2.1.1)
b2465 (tktB) -0.3
b2935 (tktA) -3.5
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
b2914 (rpiA) N.D.
b4090 (rpiB) -0.1
Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)
b3386 (rpe) -2.5
b4301 (sgcE) +0.3
Phosphoribulokinase:
D-ribulose 5-phosphate + ATP→D-ribulose-1,5-bisphosphate + ADP + H+ (EC 2.7.1.19)
b3355 (prkB) +0.2
Ribulose-bisphosphate carboxylase (in reverse):
D-ribulose-1,5-bisphosphate + CO2 + H2O→2 3-phospho-D-glycerate + 2 H+ (EC 4.1.1.39)
No genes
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
b0755 (gpmA) -0.7
b3612 (gpmI) +0.1
b4395 (ytjC) +0.1
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
b2779 (eno) N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF) +0.7
b1854 (pykA) +0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information