MetaCyc Pathway: formaldehyde assimilation II (assimilatory RuMP Cycle) in Escherichia coli BW25113

Reactions and Genes	2,4-Dinitrophenol 2 mM
6-phosphofructokinase: β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
b1723 (pfkB)	-0.3
b3916 (pfkA)	-3.3
3-hexulose-6-phosphate synthase (in reverse): formaldehyde + D-ribulose 5-phosphate→hexulose 6-phosphate (EC 4.1.2.43)
No genes
Fructose-bisphosphate aldolase: β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
b2097 (b2097)	-0.3
b2925 (fbaA)	N.D.
6-phospho-3-hexuloisomerase: hexulose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.27)
No genes
Transketolase (in reverse): β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-erythrose 4-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
b2465 (tktB)	-0.3
b2935 (tktA)	-3.5
Transaldolase (in reverse): D-erythrose 4-phosphate + β-D-fructofuranose 6-phosphate→D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate (EC 2.2.1.2)
b0008 (talB)	-1.3
b0825 (mipB)	+0.1
b2464 (talA)	-0.4
b3946 (fsaB)	-0.1
Transketolase: D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-ribose 5-phosphate (EC 2.2.1.1)
b2465 (tktB)	-0.3
b2935 (tktA)	-3.5
Ribose-5-phosphate isomerase: D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
b2914 (rpiA)	N.D.
b4090 (rpiB)	-0.1
Ribulose-phosphate 3-epimerase (in reverse): D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)
b3386 (rpe)	-2.5
b4301 (sgcE)	+0.3

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information