MetaCyc Pathway: L-citrulline biosynthesis in Escherichia coli BL21

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Reactions and Genes Mixed culture, Halobacteriovorax-10
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Arginase:
L-arginine + H2O→L-ornithine + urea
(EC 3.5.3.1)
No genes
Glutamate synthase (NADPH):
L-glutamine + H2O→L-glutamate + ammonia + H+
(EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
ECD_00036 -1.1
ECD_00037 -1.3
ECD_00436 -0.1
ECD_00631 -0.1
ECD_01237 +0.1
ECD_01238 -0.7
ECD_01481 +0.2
ECD_01782 -0.1
ECD_02359 +0.1
ECD_02399 -1.6
ECD_02500 -0.4
ECD_02625 N.D.
ECD_02719 +0.1
ECD_03077 -1.2
ECD_03078 -1.0
ECD_03211 -0.1
L-proline[in] + an electron-transfer quinone→(S)-1-pyrroline-5-carboxylate[in] + an electron-transfer quinol + H+[in] (EC 1.5.5.2)
ECD_01017 +0.1
Glutamate 5-kinase:
L-glutamate + ATP→γ-L-glutamyl 5-phosphate + ADP
(EC 2.7.2.11)
ECD_00239 -1.4
(S)-1-pyrroline-5-carboxylate + H+ + H2O→L-glutamate 5-semialdehyde (spontaneous)
No genes
Glutamate-5-semialdehyde dehydrogenase (in reverse):
γ-L-glutamyl 5-phosphate + NADPH + H+→L-glutamate 5-semialdehyde + NADP+ + phosphate
(EC 1.2.1.41)
ECD_00240 -1.8
Ornithine aminotransferase (in reverse):
L-glutamate + L-glutamate 5-semialdehyde→L-ornithine + 2-oxoglutarate
(EC 2.6.1.13)
No genes
Ornithine carbamoyltransferase:
L-ornithine + carbamoyl phosphate→L-citrulline + H+ + phosphate
(EC 2.1.3.3)
ECD_04120 -1.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information