MetaCyc Pathway: ammonia assimilation cycle III in Escherichia coli BL21

Reactions and Genes	Mixed culture, Halobacteriovorax-10
	remove
Glutamate synthase (NADPH): L-glutamine + H₂O→ammonia + L-glutamate + H⁺ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
ECD_00036	-1.1
ECD_00037	-1.3
ECD_00436	-0.1
ECD_00631	-0.1
ECD_01237	+0.1
ECD_01238	-0.7
ECD_01481	+0.2
ECD_01782	-0.1
ECD_02359	+0.1
ECD_02399	-1.6
ECD_02500	-0.4
ECD_02625	N.D.
ECD_02719	+0.1
ECD_03077	-1.2
ECD_03078	-1.0
ECD_03211	-0.1
Glutamate synthase (NADPH) (in reverse): ammonia + 2-oxoglutarate + NADPH + 2 H⁺→L-glutamate + NADP⁺ + H₂O (EC 1.4.1.13; 1.4.1.14)
ECD_02359	+0.1
ECD_02500	-0.4
ECD_02719	+0.1
ECD_03077	-1.2
ECD_03078	-1.0
Glutamate--ammonia ligase: L-glutamate + ammonium + ATP→L-glutamine + ADP + H⁺ + phosphate (EC 6.3.1.2)
ECD_03755	-1.6

Links:

Pathway details at MetaCyc
Fitness data for 17 genes
Select organism:
All MetaCyc pathways for Escherichia coli BL21

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information