MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Pseudomonas putida KT2440

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Reactions and Genes L-tyrosine 5 mM (C)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
PP_0486 -0.1
PP_0858 -0.1
PP_1109 +0.1
PP_1872 -3.4
PP_2642 +0.1
PP_2948 -0.0
PP_3544 -0.1
PP_3750 -0.1
PP_3786 -0.2
PP_4197 -1.1
PP_5275 -0.1
PP_5342 +0.0
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
PP_4473 N.D.
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
PP_1147 -0.5
PP_1989 N.D.
PP_1992 -0.2
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
PP_0664 -0.1
PP_1470 N.D.
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
PP_0121 N.D.
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
PP_0662 +0.2
PP_1471 N.D.
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
PP_3191 +0.1
PP_3446 +0.1
PP_4430 -0.1
PP_5149 -0.0
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
PP_1308 +0.0
PP_2009 -0.0
PP_3191 +0.1
PP_3446 +0.1
PP_4430 -0.1
PP_4594 +0.3
PP_5149 -0.0
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
PP_1308 +0.0
PP_2009 -0.0
PP_3191 +0.1
PP_3446 +0.1
PP_4430 -0.1
PP_4594 +0.3
PP_5149 -0.0
PP_5303 -3.1
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
PP_1157 N.D.
PP_1394 +0.1
PP_3365 +1.2
PP_3723 -0.3
PP_4679 -0.5
PP_4680 +0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
PP_4678 -0.3
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
PP_5128 -0.2
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
PP_3511 +0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information