MetaCyc Pathway: L-phenylalanine degradation VI (reductive Stickland reaction) in Pseudomonas putida KT2440

Reactions and Genes	mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
Aspartate transaminase: L-phenylalanine + 2-oxoglutarate→3-phenyl-2-oxopropanoate + L-glutamate (EC 2.6.1.1; 2.6.1.27; 2.6.1.57)
PP_0486	-0.1
PP_0858	-0.0
PP_0967	-0.5
PP_1109	+0.9
PP_1872	-0.3
PP_1972	-0.1
PP_2642	-0.1
PP_2948	-0.0
PP_3544	-0.0
PP_3590	-2.1
PP_3750	+0.2
PP_3786	-0.2
PP_4197	-4.9
PP_5275	+0.0
PP_5342	+0.1
Indolelactate dehydrogenase (in reverse): 3-phenyl-2-oxopropanoate + NADH + H+→(R)-3-(phenyl)lactate + NAD+ (EC 1.1.1.110)
No genes
Cinnamoyl-CoA:phenyllactate CoA-transferase: (R)-3-(phenyl)lactate + (E)-cinnamoyl-CoA→(R)-3-(phenyl)lactoyl-CoA + trans-cinnamate (EC 2.8.3.17)
No genes
trans-cinnamate + a reduced electron-transfer flavoprotein→3-phenylpropanoate + an oxidized electron-transfer flavoprotein + H+ (EC 1.3.8.15)
No genes
(R)-3-(phenyl)lactoyl-CoA→(E)-cinnamoyl-CoA + H2O (EC 4.2.1.175)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information