MetaCyc Pathway: C4 photosynthetic carbon assimilation cycle, PEPCK type in Pseudomonas putida KT2440

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Reactions and Genes mixed (C)s Trisodium citrate 10 mM and 4-azido-L-phenylalanine 10 mM
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Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
PP_0100 N.D.
Phosphoenolpyruvate carboxylase (in reverse):
hydrogen carbonate + phosphoenolpyruvate→oxaloacetate + phosphate (EC 4.1.1.31)
PP_1505 +0.2
Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
PP_0486 -0.1
PP_0858 -0.0
PP_1109 +0.9
PP_1872 -0.3
PP_2642 -0.1
PP_2948 -0.0
PP_3544 -0.0
PP_3750 +0.2
PP_3786 -0.2
PP_4197 -4.9
PP_5275 +0.0
PP_5342 +0.1
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) (in reverse):
oxaloacetate + NADPH + H+→(S)-malate + NADP+ (EC 1.1.1.40; 1.1.1.82)
PP_5085 -0.2
L-aspartate[in]→L-aspartate[out]
No genes
(S)-malate[in]→(S)-malate[out]
No genes
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)):
(S)-malate + NADP+→CO2 + pyruvate + NADPH (EC 1.1.1.40)
PP_5085 -0.2
Aspartate transaminase:
L-aspartate + 2-oxoglutarate→oxaloacetate + L-glutamate (EC 2.6.1.1)
PP_0486 -0.1
PP_0858 -0.0
PP_1109 +0.9
PP_1872 -0.3
PP_2642 -0.1
PP_2948 -0.0
PP_3544 -0.0
PP_3750 +0.2
PP_3786 -0.2
PP_4197 -4.9
PP_5275 +0.0
PP_5342 +0.1
Alanine transaminase (in reverse):
pyruvate + L-glutamate→L-alanine + 2-oxoglutarate (EC 2.6.1.2)
PP_0817 +0.1
PP_1872 -0.3
Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→CO2 + phosphoenolpyruvate + ADP (EC 4.1.1.49)
No genes
L-alanine[in]→L-alanine[out]
No genes
phosphoenolpyruvate[in]→phosphoenolpyruvate[out]
No genes
Alanine transaminase (in reverse):
L-alanine + 2-oxoglutarate→pyruvate + L-glutamate (EC 2.6.1.2)
PP_1872 -0.3
Pyruvate, phosphate dikinase:
pyruvate + ATP + phosphate→phosphoenolpyruvate + AMP + diphosphate + H+ (EC 2.7.9.1)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information