MetaCyc Pathway: phenylacetate degradation I (aerobic) in Pseudomonas putida KT2440

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Reactions and Genes mixed (C)s Trisodium citrate 10 mM and L-tyrosine 5 mM
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Phenylacetate--CoA ligase:
phenylacetate + ATP + coenzyme A→phenylacetyl-CoA + AMP + diphosphate (EC 6.2.1.30)
PP_3279 +0.3
phenylacetyl-CoA + NADPH + dioxygen + H+→2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + NADP+ + H2O (EC 1.14.13.149)
PP_3274 +0.1
PP_3276 -0.2
PP_3277 -0.2
PP_3278 -0.2
2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA→2-oxepin-2(3H)-ylideneacetyl-CoA (EC 5.3.3.18)
PP_3283 +0.2
2-oxepin-2(3H)-ylideneacetyl-CoA + H2O→3-oxo-5,6-didehydrosuberyl-CoA semialdehyde (EC 3.3.2.12)
PP_3270 -0.1
3-oxo-5,6-didehydrosuberyl-CoA semialdehyde + NADP+ + H2O→3-oxo-5,6-didehydrosuberyl-CoA + NADPH + 2 H+ (EC 1.2.1.91)
PP_3270 -0.1
3-oxo-5,6-didehydrosuberyl-CoA + coenzyme A→(2E)-5-carboxy-2-pentenoyl-CoA + acetyl-CoA (EC 2.3.1.223)
PP_3280 +0.2
Enoyl-CoA hydratase (in reverse):
(2E)-5-carboxy-2-pentenoyl-CoA + H2O→(3S)-hydroxyadipyl-CoA (EC 4.2.1.17)
PP_1412 +0.1
PP_1845 -0.0
PP_2047 +0.0
PP_2136 -0.0
PP_2217 +0.4
PP_3283 +0.2
PP_3284 -0.4
PP_3358 +0.0
PP_3491 -0.0
PP_3726 -0.5
PP_3732 +0.2
PP_4030 -0.0
3-hydroxyacyl-CoA dehydrogenase:
(3S)-hydroxyadipyl-CoA + NAD+→3-oxoadipoyl-CoA + NADH + H+ (EC 1.1.1.35)
PP_2047 +0.0
PP_2136 -0.0
3-oxoadipyl-CoA thiolase (in reverse):
3-oxoadipoyl-CoA + coenzyme A→succinyl-CoA + acetyl-CoA (EC 2.3.1.174)
PP_1377 -2.0
PP_3280 +0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information