MetaCyc Pathway: lipid A-core biosynthesis (E. coli K-12) in Escherichia coli BW25113

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Reactions and Genes 3 hr rep1_Tiberius_100fold_dilution
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ADP-L-glycero-β-D-manno-heptose + α-D-Kdo-(2→4)-α-D-Kdo-(2→6)-lipid A (E. coli)→α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ADP + H+ (EC 2.4.99.23)
b3621 (rfaC) -3.4
ADP-L-glycero-β-D-manno-heptose + α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli)→α-Hep-(1→3)-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ADP + H+ (EC 2.4.99.24)
b3620 (rfaF) -2.8
α-Hep-(1→3)-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ATP→α-Hep-(1→3)-4-O-phospho-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli) + ADP + H+ (EC 2.7.1.235)
b3630 (rfaP) -2.0
ADP-L-glycero-β-D-manno-heptose + α-Hep-(1→3)-4-O-phospho-α-Hep-(1→5)-[α-Kdo-(2→4)]-α-Kdo-(2→6)-lipid A (E. coli)→(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + ADP + H+ (EC 2.4.99.25)
b3632 (rfaQ) +0.1
(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + UDP-α-D-glucose→glucosyl-(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + H+ + UDP (EC 2.4.1.M73)
b3631 (rfaG) -1.8
glucosyl-(heptosyl)3-Kdo2-lipid A-phosphate (E. coli) + ATP→glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + ADP + H+
b3625 (rfaY) -1.1
glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + UDP-α-D-galactose→galactosyl-(1→6)-glucosyl-(1→3)-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + H+ + UDP
b3628 (rfaB) -0.6
Lipopolysaccharide glucosyltransferase II:
UDP-α-D-glucose + galactosyl-(1→6)-glucosyl-(1→3)-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli)→galactosyl-(glucosyl)2-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + H+ + UDP (EC 2.4.1.73)
b3627 (rfaI) -0.8
Lipopolysaccharide glucosyltransferase I:
UDP-α-D-glucose + galactosyl-(glucosyl)2-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli)→galactosyl-(glucosyl)3-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli) + H+ + UDP (EC 2.4.1.58)
b3626 (rfaJ) -1.5
ADP-L-glycero-β-D-manno-heptose + galactosyl-(glucosyl)3-(heptosyl)3-Kdo2-lipid A-bisphosphate (E. coli)→lipid A-core oligosaccharide (E. coli K-12 core type) + ADP + H+ (EC 2.4.1.M47)
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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information