MetaCyc Pathway: (5Z)-dodecenoate biosynthesis I in Phocaeicola dorei CL03T12C01

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Reactions and Genes polygalacturonic; autoclaved 2.5 mg/ml (C)
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3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H2O
(EC 4.2.1.59)
ABI39_RS00505 N.D.
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dec-2-enoyl-[acp]→a (3Z)-dec-3-enoyl-[acp]
(EC 5.3.3.14)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxododec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2
(EC 2.3.1.179; 2.3.1.41)
ABI39_RS06325 N.D.
ABI39_RS06480 +0.1
ABI39_RS06490 -0.0
ABI39_RS06540 -0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxododec-5-enoyl-[acp] + NADPH + H+→a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp] + NADP+
(EC 1.1.1.100)
ABI39_RS02135 N.D.
ABI39_RS03255 +0.1
ABI39_RS03550 +0.2
ABI39_RS06475 -0.3
ABI39_RS06545 -0.2
ABI39_RS11075 +2.2
ABI39_RS11985 +0.2
ABI39_RS16765 +0.0
ABI39_RS19260 +0.5
ABI39_RS19330 +0.0
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp]→a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + H2O
(EC 4.2.1.59)
ABI39_RS00505 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + NADH + H+→a (5Z)-dodec-5-enoyl-[acp] + NAD+
(EC 1.3.1.9)
ABI39_RS10085 -1.3
ABI39_RS14315 -0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information