MetaCyc Pathway: anteiso-branched-chain fatty acid biosynthesis in Agrobacterium fabrum C58

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Reactions and Genes spermidine (N)
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
Atu0871 -0.2
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + a malonyl-[acp] + H+→a (4S)-4-methyl-3-oxohhexanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.300)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (4S)-4-methyl-3-oxohhexanoyl-[acp] + NADPH + H+→a (4S,3R)-3-hydroxy-4-methylhexanoyl-[acp] + NADP+ (EC 1.1.1.100)
Atu0705 +0.1
Atu0990 -0.2
Atu1095 N.D.
Atu1255 +0.1
Atu1338 +0.1
Atu2042 +0.2
Atu3051 -0.1
Atu3074 -0.1
Atu3333 -0.2
Atu3531 +0.1
Atu3813 -0.2
Atu4220 -0.0
Atu4325 +0.1
Atu4595 -0.1
Atu4821 -0.1
Atu4877 -0.4
Atu5122 +1.3
Atu5214 N.D.
Atu5250 N.D.
Atu5438 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (4S,3R)-3-hydroxy-4-methylhexanoyl-[acp]→a (2E,4S)-4-methylhex-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,4S)-4-methylhex-2-enoyl-[acp] + NADH + H+→a (4S)-4-methylhexanoyl-[acp] + NAD+ (EC 1.3.1.9)
Atu0149 -0.4
Atu0757 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,4S)-4-methylhex-2-enoyl-[acp] + NADPH + H+→a (4S)-4-methylhexanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
Atu5181 -0.1
Atu5185 +0.4
Atu5391 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (4S)-4-methylhexanoyl-[acp] + a malonyl-[acp] + H+→a (6S)-6-methyl-3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Atu1097 N.D.
Atu1596 N.D.
Atu1597 N.D.
Atu4216 -0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (6S)-6-methyl-3-oxooctanoyl-[acp] + NADPH + H+→a (3R,6S)-3-hydroxy-6-methyloctanoyl-[acp] + NADP+ (EC 1.1.1.100)
Atu0705 +0.1
Atu0990 -0.2
Atu1095 N.D.
Atu1255 +0.1
Atu1338 +0.1
Atu2042 +0.2
Atu3051 -0.1
Atu3074 -0.1
Atu3333 -0.2
Atu3531 +0.1
Atu3813 -0.2
Atu4220 -0.0
Atu4325 +0.1
Atu4595 -0.1
Atu4821 -0.1
Atu4877 -0.4
Atu5122 +1.3
Atu5214 N.D.
Atu5250 N.D.
Atu5438 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,6S)-3-hydroxy-6-methyloctanoyl-[acp]→a (2E,6S)-6-methyloct-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,6S)-6-methyloct-2-enoyl-[acp] + NADH + H+→a (6S)-6-methyloctanoyl-[acp] + NAD+ (EC 1.3.1.9)
Atu0149 -0.4
Atu0757 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,6S)-6-methyloct-2-enoyl-[acp] + NADPH + H+→a (6S)-6-methyloctanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Atu5181 -0.1
Atu5185 +0.4
Atu5391 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (6S)-6-methyloctanoyl-[acp] + a malonyl-[acp] + H+→an (8S)-8-methyl-3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Atu1097 N.D.
Atu1596 N.D.
Atu1597 N.D.
Atu4216 -0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an (8S)-8-methyl-3-oxodecanoyl-[acp] + NADPH + H+→a (3R,8S)-3-hydroxy-8-methyldecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Atu0705 +0.1
Atu0990 -0.2
Atu1095 N.D.
Atu1255 +0.1
Atu1338 +0.1
Atu2042 +0.2
Atu3051 -0.1
Atu3074 -0.1
Atu3333 -0.2
Atu3531 +0.1
Atu3813 -0.2
Atu4220 -0.0
Atu4325 +0.1
Atu4595 -0.1
Atu4821 -0.1
Atu4877 -0.4
Atu5122 +1.3
Atu5214 N.D.
Atu5250 N.D.
Atu5438 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,8S)-3-hydroxy-8-methyldecanoyl-[acp]→a (2E,8S)-8-methyldec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,8S)-8-methyldec-2-enoyl-[acp] + NADH + H+→an (8S)-8-methyldecanoyl-[acyl-carrier protein] + NAD+ (EC 1.3.1.9)
Atu0149 -0.4
Atu0757 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,8S)-8-methyldec-2-enoyl-[acp] + NADPH + H+→an (8S)-8-methyldecanoyl-[acyl-carrier protein] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Atu5181 -0.1
Atu5185 +0.4
Atu5391 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
an (8S)-8-methyldecanoyl-[acyl-carrier protein] + a malonyl-[acp] + H+→a (10S)-10-methyl-3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Atu1097 N.D.
Atu1596 N.D.
Atu1597 N.D.
Atu4216 -0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (10S)-10-methyl-3-oxododecanoyl-[acp] + NADPH + H+→a (3R,10S)-3-hydroxy-10-methyldodecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Atu0705 +0.1
Atu0990 -0.2
Atu1095 N.D.
Atu1255 +0.1
Atu1338 +0.1
Atu2042 +0.2
Atu3051 -0.1
Atu3074 -0.1
Atu3333 -0.2
Atu3531 +0.1
Atu3813 -0.2
Atu4220 -0.0
Atu4325 +0.1
Atu4595 -0.1
Atu4821 -0.1
Atu4877 -0.4
Atu5122 +1.3
Atu5214 N.D.
Atu5250 N.D.
Atu5438 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,10S)-3-hydroxy-10-methyldodecanoyl-[acp]→a (2E,10S)-10-methyldodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,10S)-10-methyldodec-2-enoyl-[acp] + NADH + H+→a (10S)-10-methyldodecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Atu0149 -0.4
Atu0757 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,10S)-10-methyldodec-2-enoyl-[acp] + NADPH + H+→a (10S)-10-methyldodecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Atu5181 -0.1
Atu5185 +0.4
Atu5391 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (10S)-10-methyldodecanoyl-[acp] + a malonyl-[acp] + H+→a (12S)-12-methyl-3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Atu1097 N.D.
Atu1596 N.D.
Atu1597 N.D.
Atu4216 -0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (12S)-12-methyl-3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R,12S)-3-hydroxy-12-methyltetradecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Atu0705 +0.1
Atu0990 -0.2
Atu1095 N.D.
Atu1255 +0.1
Atu1338 +0.1
Atu2042 +0.2
Atu3051 -0.1
Atu3074 -0.1
Atu3333 -0.2
Atu3531 +0.1
Atu3813 -0.2
Atu4220 -0.0
Atu4325 +0.1
Atu4595 -0.1
Atu4821 -0.1
Atu4877 -0.4
Atu5122 +1.3
Atu5214 N.D.
Atu5250 N.D.
Atu5438 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,12S)-3-hydroxy-12-methyltetradecanoyl-[acp]→a (2E,12S)-12-methyltetradec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,12S)-12-methyltetradec-2-enoyl-[acp] + NADH + H+→a (12S)-12-methyltetradecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Atu0149 -0.4
Atu0757 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,12S)-12-methyltetradec-2-enoyl-[acp] + NADPH + H+→a (12S)-12-methyltetradecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
Atu5181 -0.1
Atu5185 +0.4
Atu5391 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (12S)-12-methyltetradecanoyl-[acp] + a malonyl-[acp] + H+→a (14S)-14-methyl-3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
Atu1097 N.D.
Atu1596 N.D.
Atu1597 N.D.
Atu4216 -0.0
Oleoyl-[acyl-carrier-protein] hydrolase:
a (12S)-12-methyltetradecanoyl-[acp] + H2O→(12S)-12-methyltetradecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (14S)-14-methyl-3-oxohexadecanoyl-[acp] + NADPH + H+→a (3R,14S)-3-hydroxy-14-methylhexadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
Atu0705 +0.1
Atu0990 -0.2
Atu1095 N.D.
Atu1255 +0.1
Atu1338 +0.1
Atu2042 +0.2
Atu3051 -0.1
Atu3074 -0.1
Atu3333 -0.2
Atu3531 +0.1
Atu3813 -0.2
Atu4220 -0.0
Atu4325 +0.1
Atu4595 -0.1
Atu4821 -0.1
Atu4877 -0.4
Atu5122 +1.3
Atu5214 N.D.
Atu5250 N.D.
Atu5438 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,14S)-3-hydroxy-14-methylhexadecanoyl-[acp]→a (2E,14S)-14-methylhexadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,14S)-14-methylhexadec-2-enoyl-[acp] + NADH + H+→a (14S)-14-methylhexadecanoyl-[acp] + NAD+ (EC 1.3.1.9)
Atu0149 -0.4
Atu0757 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E,14S)-14-methylhexadec-2-enoyl-[acp] + NADPH + H+→a (14S)-14-methylhexadecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
Atu5181 -0.1
Atu5185 +0.4
Atu5391 N.D.
Oleoyl-[acyl-carrier-protein] hydrolase:
a (14S)-14-methylhexadecanoyl-[acp] + H2O→(14S)-14-methylhexadecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information