MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Pseudomonas sp. RS175

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Reactions and Genes Ferulic Acid (C) 2.5 mM
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
PFR28_03474 -0.0
PFR28_03698 -2.4
PFR28_03699 -3.3
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
PFR28_03697 -3.0
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
PFR28_01579 +0.1
PFR28_04278 -3.2
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
PFR28_00420 -2.1
PFR28_00654 -0.6
PFR28_01820 -0.8
PFR28_01972 -0.1
PFR28_02982 +0.2
PFR28_04050 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information