MetaCyc Pathway: oleate β-oxidation in Agrobacterium fabrum C58

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Reactions and Genes L-Lysine (N)
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Long-chain-fatty-acid--CoA ligase:
oleate + ATP + coenzyme A→oleoyl-CoA + AMP + diphosphate (EC 6.2.1.3)
Atu0405 -0.1
oleoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,9Z)-octadec-2,9-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Enoyl-CoA hydratase (in reverse):
(2E,9Z)-octadec-2,9-enoyl-CoA + H2O→(S)-3-hydroxy-(9Z)-octadecenoyl-CoA (EC 4.2.1.17)
Atu0322 -0.8
Atu0503 +0.2
Atu0733 +0.1
Atu1417 +0.1
Atu3481 -0.2
Atu3505 -0.2
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(9Z)-octadecenoyl-CoA + NAD+→3-oxo-(9Z)-octadecenoyl-CoA + NADH + H+ (EC 1.1.1.35)
Atu0503 +0.2
Atu1415 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(9Z)-octadecenoyl-CoA + coenzyme A→acetyl-CoA + (7Z)-hexadecenoyl-CoA (EC 2.3.1.16)
Atu0502 -0.0
Atu2769 -3.0
Atu3475 -0.2
Atu4549 -0.2
Atu4823 -0.3
(7Z)-hexadecenoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,7Z)-hexadec-2,7-dienoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Enoyl-CoA hydratase (in reverse):
(2E,7Z)-hexadec-2,7-dienoyl-CoA + H2O→(S)-3-hydroxy-(7Z)-hexadecenoyl-CoA (EC 4.2.1.17)
Atu0322 -0.8
Atu0503 +0.2
Atu0733 +0.1
Atu1417 +0.1
Atu3481 -0.2
Atu3505 -0.2
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(7Z)-hexadecenoyl-CoA + NAD+→3-oxo-(7Z)-hexadecenoyl-CoA + NADH + H+ (EC 1.1.1.35)
Atu0503 +0.2
Atu1415 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(7Z)-hexadecenoyl-CoA + coenzyme A→acetyl-CoA + (5Z)-tetradecenoyl-CoA (EC 2.3.1.16)
Atu0502 -0.0
Atu2769 -3.0
Atu3475 -0.2
Atu4549 -0.2
Atu4823 -0.3
(5Z)-tetradecenoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E,5Z)-tetradec-2,5-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Dodecenoyl-CoA isomerase:
(2E,5Z)-tetradec-2,5-enoyl-CoA→(3E,5Z)-tetradeca-3,5-dienoyl-CoA (EC 5.3.3.8)
No genes
Enoyl-CoA hydratase (in reverse):
(2E,5Z)-tetradec-2,5-enoyl-CoA + H2O→(S)-3-hydroxy-(5Z)-tetradecenoyl-CoA (EC 4.2.1.17)
Atu0322 -0.8
Atu0503 +0.2
Atu0733 +0.1
Atu1417 +0.1
Atu3481 -0.2
Atu3505 -0.2
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-(5Z)-tetradecenoyl-CoA + NAD+→3-oxo-(5Z)-tetradecenoyl-CoA + NADH + H+ (EC 1.1.1.211; 1.1.1.35)
Atu0503 +0.2
Atu1415 +0.0
(3E,5Z)-tetradeca-3,5-dienoyl-CoA + H2O→(3E,5Z)-tetradeca-3,5-dienoate + coenzyme A + H+
Atu2756 -0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-(5Z)-tetradecenoyl-CoA + coenzyme A→acetyl-CoA + (3Z)-dodec-3-enoyl-CoA (EC 2.3.1.16)
Atu0502 -0.0
Atu2769 -3.0
Atu3475 -0.2
Atu4549 -0.2
Atu4823 -0.3
Dodecenoyl-CoA isomerase:
(3Z)-dodec-3-enoyl-CoA→(2E)-dodec-2-enoyl-CoA (EC 5.3.3.8)
No genes
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-dodec-2-enoyl-CoA + H2O→(S)-3-hydroxydodecanoyl-CoA (EC 4.2.1.74)
No genes
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxydodecanoyl-CoA + NAD+→3-oxododecanoyl-CoA + NADH + H+ (EC 1.1.1.211; 1.1.1.35)
Atu0503 +0.2
Atu1415 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxododecanoyl-CoA + coenzyme A→acetyl-CoA + decanoyl-CoA (EC 2.3.1.16)
Atu0502 -0.0
Atu2769 -3.0
Atu3475 -0.2
Atu4549 -0.2
Atu4823 -0.3
decanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-dec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
Atu0501 +0.0
Atu0732 +0.1
Atu1310 N.D.
Atu1414 +0.1
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-dec-2-enoyl-CoA + H2O→(S)-3-hydroxydecanoyl-CoA (EC 4.2.1.74)
Atu0733 +0.1
Atu1417 +0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxydecanoyl-CoA + NAD+→3-oxodecanoyl-CoA + NADH + H+ (EC 1.1.1.35)
Atu0503 +0.2
Atu1415 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxodecanoyl-CoA + coenzyme A→acetyl-CoA + octanoyl-CoA (EC 2.3.1.16)
Atu0502 -0.0
Atu2769 -3.0
Atu3475 -0.2
Atu4549 -0.2
Atu4823 -0.3
octanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-oct-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
Atu0501 +0.0
Atu0732 +0.1
Atu1310 N.D.
Atu1414 +0.1
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-oct-2-enoyl-CoA + H2O→(3S)-3-hydroxyoctanoyl-CoA (EC 4.2.1.74)
No genes
3-hydroxyacyl-CoA dehydrogenase:
(3S)-3-hydroxyoctanoyl-CoA + NAD+→3-oxooctanoyl-CoA + NADH + H+ (EC 1.1.1.35)
Atu0503 +0.2
Atu1415 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxooctanoyl-CoA + coenzyme A→acetyl-CoA + hexanoyl-CoA (EC 2.3.1.16)
Atu0502 -0.0
Atu2769 -3.0
Atu3475 -0.2
Atu4549 -0.2
Atu4823 -0.3
hexanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-hex-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
Atu0501 +0.0
Atu0732 +0.1
Atu1310 N.D.
Atu1414 +0.1
Long-chain-enoyl-CoA hydratase (in reverse):
(2E)-hex-2-enoyl-CoA + H2O→(S)-3-hydroxyhexanoyl-CoA (EC 4.2.1.74)
Atu0733 +0.1
Atu1417 +0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxyhexanoyl-CoA + NAD+→3-oxohexanoyl-CoA + NADH + H+ (EC 1.1.1.35)
Atu0503 +0.2
Atu1415 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
3-oxohexanoyl-CoA + coenzyme A→acetyl-CoA + butanoyl-CoA (EC 2.3.1.16; 2.3.1.9)
Atu0502 -0.0
Atu2769 -3.0
Atu3475 -0.2
Atu4549 -0.2
Atu4823 -0.3
butanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→crotonyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.1)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
Atu0733 +0.1
Atu1417 +0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
Atu0503 +0.2
Atu1415 +0.0
Atu3597 -1.4
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
Atu0502 -0.0
Atu2769 -3.0
Atu3475 -0.2
Atu4549 -0.2
Atu4823 -0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information