MetaCyc Pathway: (S)-lactate fermentation to propanoate, acetate and hydrogen in Echinicola vietnamensis KMM 6221, DSM 17526

Reactions and Genes	L-Methionine (N)
L-lactate dehydrogenase: (S)-lactate + NAD+→pyruvate + NADH + H+ (EC 1.1.1.27)
No genes
Pyruvate carboxylase: pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
Echvi_3962	-0.0
Formate C-acetyltransferase (in reverse): pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
No genes
formate + H+→H2 + CO2
No genes
Malate dehydrogenase (in reverse): oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
Echvi_0162	N.D.
Echvi_2076	N.D.
Phosphate acetyltransferase: acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
No genes
Acetate kinase (in reverse): acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
No genes
Fumarate hydratase: (S)-malate→fumarate + H2O (EC 4.2.1.2)
Echvi_1329	N.D.
Succinate dehydrogenase (ubiquinone): fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
succinate + propanoyl-CoA→succinyl-CoA + propanoate (EC 2.8.3.27)
No genes
Methylmalonyl-CoA mutase (in reverse): succinyl-CoA→(R)-methylmalonyl-CoA (EC 5.4.99.2)
Echvi_2441	+1.4
Echvi_4683	+0.0
Methylmalonyl-CoA epimerase: (R)-methylmalonyl-CoA→(S)-methylmalonyl-CoA (EC 5.1.99.1)
Echvi_0089	+0.4
(S)-methylmalonyl-CoA[in] + Na+[in] + H+[out]→propanoyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.3)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information