MetaCyc Pathway: glycine betaine degradation III in Pseudomonas simiae WCS417

Reactions and Genes	D-Glucosamine Hydrochloride (C)
	remove
glycine betaine + NADH + dioxygen + H⁺→N,N-dimethylglycine + formaldehyde + NAD⁺ + H₂O (EC 1.14.13.251)
PS417_26215	+0.5
PS417_26220	-0.2
N,N-dimethylglycine + 2 an oxidized ferredoxin [iron-sulfur] cluster + H₂O→sarcosine + formaldehyde + 2 H⁺ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.5.7.3)
PS417_26240	+0.2
sarcosine + 2 an oxidized ferredoxin [iron-sulfur] cluster + H₂O→glycine + formaldehyde + 2 H⁺ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.5.7.3)
PS417_26240	+0.2
Glycine hydroxymethyltransferase (in reverse): glycine + a 5,10-methylenetetrahydrofolate + H₂O→L-serine + a tetrahydrofolate (EC 2.1.2.1)
PS417_24275	+0.0
PS417_24280	N.D.
PS417_26195	+0.2
L-serine ammonia-lyase: L-serine→2-aminoprop-2-enoate + H₂O (EC 4.3.1.17)
PS417_05075	-0.1
PS417_22365	+0.2
PS417_26310	-0.3
Tryptophanase: 2-aminoprop-2-enoate→2-iminopropanoate (spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
PS417_05075	-0.1
PS417_01110	-0.0
PS417_12125	-3.2
PS417_22365	+0.2
PS417_26310	-0.3
2-iminopropanoate + H₂O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
PS417_05075	-0.1
PS417_01110	-0.0
PS417_12125	-3.2
PS417_22365	+0.2
PS417_26310	-0.3
PS417_27835	-0.5

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Pathway details at MetaCyc
Fitness data for 12 genes
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All MetaCyc pathways for Pseudomonas simiae WCS417

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information