MetaCyc Pathway: (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) in Echinicola vietnamensis KMM 6221, DSM 17526

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Reactions and Genes D-Glucosamine Hydrochloride (C)
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arachidonoyl-CoA + malonyl-CoA + H+→(7Z,10Z,13Z,16Z)-3-oxodocosatetraenoyl-CoA + CO2 + coenzyme A
No genes
(7Z,10Z,13Z,16Z)-3-oxodocosatetraenoyl-CoA + NADPH + H+→(3R,7Z,10Z,13Z,16Z)-3-hydroxydocosatetraenoyl-CoA + NADP+ (EC 1.1.1.330)
No genes
(3R,7Z,10Z,13Z,16Z)-3-hydroxydocosatetraenoyl-CoA→(2E,7Z,10Z,13Z,16Z)-docosa-2,7,10,13,16-pentaenoyl-CoA + H2O (EC 4.2.1.134)
No genes
(2E,7Z,10Z,13Z,16Z)-docosa-2,7,10,13,16-pentaenoyl-CoA + NADPH + H+→adrenoyl-CoA + NADP+ (EC 1.3.1.93)
No genes
adrenoyl-CoA + malonyl-CoA + H+→(9Z,12Z,15Z,18Z)-3-oxotetracosatetraenoyl-CoA + CO2 + coenzyme A
No genes
(9Z,12Z,15Z,18Z)-3-oxotetracosatetraenoyl-CoA + NADPH + H+→(3R,9Z,12Z,15Z,18Z)-3-hydroxytetracosatetraenoyl-CoA + NADP+ (EC 1.1.1.330)
No genes
(3R,9Z,12Z,15Z,18Z)-3-hydroxytetracosatetraenoyl-CoA→(2E,9Z,12Z,15Z,18Z)-tetracosapentaenoyl-CoA + H2O (EC 4.2.1.134)
No genes
(2E,9Z,12Z,15Z,18Z)-tetracosapentaenoyl-CoA + NADPH + H+→(9Z,12Z,15Z,18Z)-tetracosatetraenoyl-CoA + NADP+ (EC 1.3.1.93)
No genes
Linoleoyl-CoA desaturase:
(9Z,12Z,15Z,18Z)-tetracosatetraenoyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(6Z,9Z,12Z,15Z,18Z)-tetracosapentaenoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
(EC 1.14.19.3)
Echvi_1008 +0.0
Echvi_2487 +0.1
Acyl-CoA oxidase:
(6Z,9Z,12Z,15Z,18Z)-tetracosapentaenoyl-CoA + dioxygen→(2E,6Z,9Z,12Z,15Z,18Z)-tetracosahexaenoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
(2E,6Z,9Z,12Z,15Z,18Z)-tetracosahexaenoyl-CoA + H2O→(3S,6Z,9Z,12Z,15Z,18Z)-3-hydroxytetracosapentaenoyl-CoA
(EC 4.2.1.17)
Echvi_0069 +0.1
Echvi_0343 -0.5
Echvi_1069 -1.3
Echvi_4069 -0.4
(3S,6Z,9Z,12Z,15Z,18Z)-3-hydroxytetracosapentaenoyl-CoA + NAD+→(6Z,9Z,12Z,15Z,18Z)-3-oxotetracosapentaenoyl-CoA + NADH + H+
No genes
Acetyl-CoA C-acyltransferase (in reverse):
(6Z,9Z,12Z,15Z,18Z)-3-oxotetracosapentaenoyl-CoA + coenzyme A→(4Z,7Z,10Z,13Z,16Z)-docosapentaenoyl-CoA + acetyl-CoA
(EC 2.3.1.16)
Echvi_1071 -0.8
Echvi_3705 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information